Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046203157> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2046203157 endingPage "306a" @default.
- W2046203157 startingPage "305a" @default.
- W2046203157 abstract "Living cells are responsive to mechanical cues from their surroundings. A primary means by which cells sense and transmit mechanical force is through integrins, a class of heterodimeric, transmembrane proteins that physically link the actin cytoskeleton to the extracellular matrix (ECM). How these proteins assemble into force sensing and transducing structures is poorly understood, due largely to a lack of methods that directly visualize the mechanical force exerted by integrins with nanometer spatial resolution. Here we use Förster resonance energy transfer (FRET)-based molecular tension sensors (MTSs) to visualize the forces exerted by integrins in living cells. Simultaneous super-resolution imaging of MTSs and GFP-tagged cellular proteins results in maps of force-producing structures within focal adhesions (FAs) with sub-diffraction spatial resolution. We find that on our Arg-Gly-Asp (RGD)-presenting surfaces αvβ3 integrin localizes to high force regions, whereas α5β1 integrin localization is more diffuse. The canonical FA proteins paxillin, vinculin, talin, and α-actinin colocalize with force production. Surprisingly, paxillin, which is not generally considered to play a direct role in force transmission, shows a higher degree of spatial correlation with force than vinculin, talin, or α-actinin, proteins with hypothesized roles in force transmission. Super-resolution imaging reveals that MTS-measured tension, paxillin, and actin form linear structures in many adhesions, suggesting that the fundamental unit of FA assembly may be an actin bundle connected to the substrate via a variety of integrin- and actin-binding complexes. The high degree of spatial correlation of both paxillin and αvβ3 integrin with mechanical tension suggests that these proteins may play direct roles in cellular mechanotransduction." @default.
- W2046203157 created "2016-06-24" @default.
- W2046203157 creator A5015903700 @default.
- W2046203157 creator A5046259156 @default.
- W2046203157 creator A5055520292 @default.
- W2046203157 creator A5057324568 @default.
- W2046203157 creator A5075755609 @default.
- W2046203157 date "2015-01-01" @default.
- W2046203157 modified "2023-09-28" @default.
- W2046203157 title "Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps" @default.
- W2046203157 doi "https://doi.org/10.1016/j.bpj.2014.11.1661" @default.
- W2046203157 hasPublicationYear "2015" @default.
- W2046203157 type Work @default.
- W2046203157 sameAs 2046203157 @default.
- W2046203157 citedByCount "0" @default.
- W2046203157 crossrefType "journal-article" @default.
- W2046203157 hasAuthorship W2046203157A5015903700 @default.
- W2046203157 hasAuthorship W2046203157A5046259156 @default.
- W2046203157 hasAuthorship W2046203157A5055520292 @default.
- W2046203157 hasAuthorship W2046203157A5057324568 @default.
- W2046203157 hasAuthorship W2046203157A5075755609 @default.
- W2046203157 hasBestOaLocation W20462031571 @default.
- W2046203157 hasConcept C116581196 @default.
- W2046203157 hasConcept C120665830 @default.
- W2046203157 hasConcept C121332964 @default.
- W2046203157 hasConcept C12554922 @default.
- W2046203157 hasConcept C125705527 @default.
- W2046203157 hasConcept C142669718 @default.
- W2046203157 hasConcept C1491633281 @default.
- W2046203157 hasConcept C151166631 @default.
- W2046203157 hasConcept C185592680 @default.
- W2046203157 hasConcept C189165786 @default.
- W2046203157 hasConcept C195687474 @default.
- W2046203157 hasConcept C2777979848 @default.
- W2046203157 hasConcept C55493867 @default.
- W2046203157 hasConcept C62478195 @default.
- W2046203157 hasConcept C86803240 @default.
- W2046203157 hasConcept C91881484 @default.
- W2046203157 hasConcept C95444343 @default.
- W2046203157 hasConcept C96305047 @default.
- W2046203157 hasConceptScore W2046203157C116581196 @default.
- W2046203157 hasConceptScore W2046203157C120665830 @default.
- W2046203157 hasConceptScore W2046203157C121332964 @default.
- W2046203157 hasConceptScore W2046203157C12554922 @default.
- W2046203157 hasConceptScore W2046203157C125705527 @default.
- W2046203157 hasConceptScore W2046203157C142669718 @default.
- W2046203157 hasConceptScore W2046203157C1491633281 @default.
- W2046203157 hasConceptScore W2046203157C151166631 @default.
- W2046203157 hasConceptScore W2046203157C185592680 @default.
- W2046203157 hasConceptScore W2046203157C189165786 @default.
- W2046203157 hasConceptScore W2046203157C195687474 @default.
- W2046203157 hasConceptScore W2046203157C2777979848 @default.
- W2046203157 hasConceptScore W2046203157C55493867 @default.
- W2046203157 hasConceptScore W2046203157C62478195 @default.
- W2046203157 hasConceptScore W2046203157C86803240 @default.
- W2046203157 hasConceptScore W2046203157C91881484 @default.
- W2046203157 hasConceptScore W2046203157C95444343 @default.
- W2046203157 hasConceptScore W2046203157C96305047 @default.
- W2046203157 hasIssue "2" @default.
- W2046203157 hasLocation W20462031571 @default.
- W2046203157 hasLocation W20462031572 @default.
- W2046203157 hasLocation W20462031573 @default.
- W2046203157 hasOpenAccess W2046203157 @default.
- W2046203157 hasPrimaryLocation W20462031571 @default.
- W2046203157 hasRelatedWork W1533959399 @default.
- W2046203157 hasRelatedWork W1571135400 @default.
- W2046203157 hasRelatedWork W2097381572 @default.
- W2046203157 hasRelatedWork W2109137926 @default.
- W2046203157 hasRelatedWork W2339276180 @default.
- W2046203157 hasRelatedWork W2529664565 @default.
- W2046203157 hasRelatedWork W2788672886 @default.
- W2046203157 hasRelatedWork W4210597571 @default.
- W2046203157 hasRelatedWork W4247056331 @default.
- W2046203157 hasRelatedWork W4250267039 @default.
- W2046203157 hasVolume "108" @default.
- W2046203157 isParatext "false" @default.
- W2046203157 isRetracted "false" @default.
- W2046203157 magId "2046203157" @default.
- W2046203157 workType "article" @default.