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• W2046204911 startingPage "204" @default.
• W2046204911 abstract "The ORF38 of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), or AcORF38, contains a conserved motif of Nudix (nucleotide diphosphate X) superfamily. It has the highest homology with ADP-ribose pyrophosphatase (ADPRase), a subfamily of Nudix pyrophosphatase. In the current study, recombinant AcORF38 protein was prepared and shown to have ADPRase activity, with a Km of 204 microM, and K(cat) of 6.96 s(-1) at pH 8.0 and 5 mM MgCl2. The transcription of AcORF38 was detected 2 h postinfection, and lasted until the late stage. An orf38 gene-deleted mutant virus, vAcGFP-Delta38, was constructed. Although it produced progeny virus, the yield of extracellular virus was less than 1% of the wild-type virus. The activity of viral very late gene promoter was also greatly reduced in vAcGFP-Delta38-infected cells as indicated by the expression of green fluorescence protein gene driven by polyhedrin promoter. The mutant phenotype was rescued by co-transfection with an AcORF38-expressing plasmid. These results suggest that AcORF38 plays an important role in virus replication, although the detail is to be elucidated." @default.
• W2046204911 created "2016-06-24" @default.
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• W2046204911 date "2007-04-01" @default.
• W2046204911 modified "2023-09-26" @default.
• W2046204911 title "AcMNPV ORF38 protein has the activity of ADP-ribose pyrophosphatase and is important for virus replication" @default.
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• W2046204911 doi "https://doi.org/10.1016/j.virol.2006.11.017" @default.
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