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• W2046208317 startingPage "465" @default.
• W2046208317 abstract "Crude biopolymer was extracted from spent yeast, lyophilized and fractionated on Sephadex G-100 to yield two fractions coded as fraction I and II. Fraction I was composed of both carbohydrates and proteins, showing emulsifying activity whereas fraction II consisted of only proteins and possessed no activity. Hence composition and chemical characterization of the purified fraction I (bioemulsifier) was analyzed using various analytical techniques. It was found that the sample contained 96% of carbohydrates consisting mainly of glucose with minor quantities of mannose, and 4% of protein built from 17 amino acids with the highest content of serine followed by alanine. The results also indicated that the sample was protein-bound glucan with the average molecular weight of 1.93×10(5) Da. The functional groups and primary structure of the sample were revealed by FT-IR and NMR techniques. The data demonstrate that the sample comprises a mixture of (1→4)-α- and (1→3)-β-D-glucans bound with protein. Enzymatic hydrolyses using α-amylase and endo 1,3-β-D-glucanase confirmed the presence of both glucans. Therefore, this bioemulsifier was identified as glucan-protein complex which is different from usual mannoprotein emulsifier derived from yeasts." @default.
• W2046208317 created "2016-06-24" @default.
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• W2046208317 date "2010-11-01" @default.
• W2046208317 modified "2023-10-16" @default.
• W2046208317 title "Characterization of an unexpected bioemulsifier from spent yeast obtained from Thai traditional liquor distillation" @default.
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• W2046208317 doi "https://doi.org/10.1016/j.ijbiomac.2010.06.013" @default.
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