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• W2046252545 abstract "To evaluate a new tenascin-C assay performed on the Bayer Immuno 1 system.The precision was measured using three levels of serum pools. Linearity was tested by diluting patient serum samples containing high tenascin-C concentrations, and the minimal detectable concentration determined by repetitive analysis of the zero calibrator. Preliminary reference intervals were determined by testing serum samples from 220 healthy individuals. Biovariability was estimated in a cohort of 20 apparently healthy subjects over 18 days. The levels of tenascin-C in patients with different liver diseases was tested.The detection limit was 2 ng/mL. At concentrations ranging from 325 to 1957 ng/mL the assay demonstrated within-run and between-run CVs ranging from 4% to 3.6% and 8.4% to 6.7%, respectively. Dilutions of sera were linear and parallel to the standard curve with recoveries ranging from 97% to 100%. The reference interval (central 95% interval) for tenascin-C in serum of healthy adults was 199-906 ng/mL. The variability study yielded an analytical variability, CV(A), of 1.8%; a within-subject variability, CV(I), of 11.7%; and a between-subject variability, CV(G), of 39.3%. Tenascin-C concentrations in sera of liver disease patients were significantly increased.The novel assay provides a rapid and reliable procedure for the determination of tenascin-C levels in human sera." @default.
• W2046252545 created "2016-06-24" @default.
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• W2046252545 date "2000-02-01" @default.
• W2046252545 modified "2023-10-17" @default.
• W2046252545 title "Enzyme immunoassay for the measurement of human tenascin-C on the Bayer Immuno 1™ analyzer" @default.
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• W2046252545 doi "https://doi.org/10.1016/s0009-9120(99)00083-1" @default.
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