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- W2046258235 abstract "Two new approaches, click-chemistry and disulfide bond bridges, to surface-immobilizing nucleic acids for single-molecule fluorescence experiments using covalent bonds and self-assembled monolayers are reported. Both approaches are specific and yield comparable results to the avidin–biotin linkage, but offer new surface-chemical properties that might be advantageous to prevent nonspecific binding of biopolymers to the surface and to expand the range of fluorescent probes that can be employed in single-molecule studies. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article." @default.
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- W2046258235 date "2009-12-04" @default.
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- W2046258235 title "Covalent-Bond-Based Immobilization Approaches for Single-Molecule Fluorescence" @default.
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- W2046258235 doi "https://doi.org/10.1002/cbic.200900640" @default.
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