FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046266902> ?p ?o ?g. }

• W2046266902 endingPage "681" @default.
• W2046266902 startingPage "674" @default.
• W2046266902 abstract "Ribosomal RNA genes are present near the end of the short arm and, to a lesser extent, near the centromere of the B chromosomes of some populations of Brachycome dichromosomatica. The internal transcribed spacer (ITS2) was amplified by PCR from total leaf DNA using primers within the conserved regions encoding the 5.8S and 25S stable rRNA species. Comparison of PCR amplified ITS2 sequences from several individual plants without B chromosomes with corresponding sequences derived from microdissected B chromosomes revealed two consistent differences between the rDNA of A and B chromosomes. One of these differences produced an SfcI restriction site that was present only in the ITS2 of the B-chromosome rDNA. Amplification by PCR of ITS2 from total genomic DNA from plants with and without B chromosomes showed an additive relationship between the amount of PCR product containing the SfcI site and the number of B chromosomes present. Quantitative analysis indicated that the proportion of total nuclear rDNA present on a single B chromosome varied between 2 and 4% in different A chromosome backgrounds. Similar experiments, with appropriate positive and negative controls, using reverse transcriptase PCR of the equivalent region within the 40S precursor rRNA, suggested that the B-chromosome rDNA was not transcribed. Similarly, PCR of reverse transcribed total RNA from plants containing B chromosomes using primers specific for the B chromosome ITS2 was unable to detect a transcript from the B chromosome. Keywords: B chromosome, ribosomal RNA genes, transcription." @default.
• W2046266902 created "2016-06-24" @default.
• W2046266902 creator A5008927557 @default.
• W2046266902 creator A5032279352 @default.
• W2046266902 creator A5038960851 @default.
• W2046266902 creator A5045178725 @default.
• W2046266902 date "1997-10-01" @default.
• W2046266902 modified "2023-09-27" @default.
• W2046266902 title "Ribosomal RNA genes specific to the B chromosomes in <i>Brachycome dichromosomatica</i> are not transcribed in leaf tissue" @default.
• W2046266902 cites W1973372004 @default.
• W2046266902 cites W1976570230 @default.
• W2046266902 cites W1980837851 @default.
• W2046266902 cites W1984731778 @default.
• W2046266902 cites W1989955580 @default.
• W2046266902 cites W1996363016 @default.
• W2046266902 cites W2001526158 @default.
• W2046266902 cites W2002199864 @default.
• W2046266902 cites W2002514402 @default.
• W2046266902 cites W2013773385 @default.
• W2046266902 cites W2017542644 @default.
• W2046266902 cites W2026727972 @default.
• W2046266902 cites W2027177712 @default.
• W2046266902 cites W2028040294 @default.
• W2046266902 cites W2035743100 @default.
• W2046266902 cites W2052049548 @default.
• W2046266902 cites W2060333964 @default.
• W2046266902 cites W2066617345 @default.
• W2046266902 cites W2071156902 @default.
• W2046266902 cites W2077276679 @default.
• W2046266902 cites W2094001628 @default.
• W2046266902 cites W2095407660 @default.
• W2046266902 cites W2110102473 @default.
• W2046266902 cites W2144736139 @default.
• W2046266902 cites W2150981906 @default.
• W2046266902 cites W227482328 @default.
• W2046266902 cites W3093014672 @default.
• W2046266902 cites W1979318667 @default.
• W2046266902 doi "https://doi.org/10.1139/g97-089" @default.
• W2046266902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9352646" @default.
• W2046266902 hasPublicationYear "1997" @default.
• W2046266902 type Work @default.
• W2046266902 sameAs 2046266902 @default.
• W2046266902 citedByCount "36" @default.
• W2046266902 countsByYear W20462669022012 @default.
• W2046266902 countsByYear W20462669022014 @default.
• W2046266902 countsByYear W20462669022015 @default.
• W2046266902 countsByYear W20462669022016 @default.
• W2046266902 countsByYear W20462669022017 @default.
• W2046266902 countsByYear W20462669022018 @default.
• W2046266902 countsByYear W20462669022019 @default.
• W2046266902 crossrefType "journal-article" @default.
• W2046266902 hasAuthorship W2046266902A5008927557 @default.
• W2046266902 hasAuthorship W2046266902A5032279352 @default.
• W2046266902 hasAuthorship W2046266902A5038960851 @default.
• W2046266902 hasAuthorship W2046266902A5045178725 @default.
• W2046266902 hasConcept C104317684 @default.
• W2046266902 hasConcept C116029586 @default.
• W2046266902 hasConcept C153911025 @default.
• W2046266902 hasConcept C155794727 @default.
• W2046266902 hasConcept C207812311 @default.
• W2046266902 hasConcept C30481170 @default.
• W2046266902 hasConcept C53226629 @default.
• W2046266902 hasConcept C54355233 @default.
• W2046266902 hasConcept C67905577 @default.
• W2046266902 hasConcept C8313540 @default.
• W2046266902 hasConcept C86803240 @default.
• W2046266902 hasConcept C90132467 @default.
• W2046266902 hasConceptScore W2046266902C104317684 @default.
• W2046266902 hasConceptScore W2046266902C116029586 @default.
• W2046266902 hasConceptScore W2046266902C153911025 @default.
• W2046266902 hasConceptScore W2046266902C155794727 @default.
• W2046266902 hasConceptScore W2046266902C207812311 @default.
• W2046266902 hasConceptScore W2046266902C30481170 @default.
• W2046266902 hasConceptScore W2046266902C53226629 @default.
• W2046266902 hasConceptScore W2046266902C54355233 @default.
• W2046266902 hasConceptScore W2046266902C67905577 @default.
• W2046266902 hasConceptScore W2046266902C8313540 @default.
• W2046266902 hasConceptScore W2046266902C86803240 @default.
• W2046266902 hasConceptScore W2046266902C90132467 @default.
• W2046266902 hasIssue "5" @default.
• W2046266902 hasLocation W20462669021 @default.
• W2046266902 hasLocation W20462669022 @default.
• W2046266902 hasOpenAccess W2046266902 @default.
• W2046266902 hasPrimaryLocation W20462669021 @default.
• W2046266902 hasRelatedWork W2053368601 @default.
• W2046266902 hasRelatedWork W2075449180 @default.
• W2046266902 hasRelatedWork W2076061307 @default.
• W2046266902 hasRelatedWork W2092742244 @default.
• W2046266902 hasRelatedWork W2140298376 @default.
• W2046266902 hasRelatedWork W2282743844 @default.
• W2046266902 hasRelatedWork W2383830938 @default.
• W2046266902 hasRelatedWork W2394236990 @default.
• W2046266902 hasRelatedWork W2808989341 @default.
• W2046266902 hasRelatedWork W4239664630 @default.
• W2046266902 hasVolume "40" @default.
• W2046266902 isParatext "false" @default.
• W2046266902 isRetracted "false" @default.
• W2046266902 magId "2046266902" @default.

• next