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• W2046333852 abstract "Blood B lymphocytes obtained from Plasmodium falciparum-immune Saimiri monkeys were assayed for their in vitro differentiation in immunoglobulin-secreting cells upon restimulation with P. falciparum-parasitized Saimiri red blood cells. Selected culture conditions enabled appropriately stimulated blood B cells to secrete 3F11/G10+ IgG, detected in the supernatants by means of a dot immunobinding assay. Primed blood B lymphocytes from P. falciparum-immune Saimiri monkeys were thus able to secrete IgG when restimulated by parasitized red blood cells in the presence of T cell- and monocyte-derived cytokines (recombinant human cytokines). These primed blood B cells, which were able to differentiate, were shown to secrete antibodies reactive with P. falciparum-infected red blood cells, as detected by means of an indirect immunofluorescence assay, and reactive with P. falciparum-infected red blood cell extracts, as detected by means of Western blot analysis. Furthermore, due to the possibility of discriminating between IgG subtypes in the squirrel monkey (3F11/G10+ ::3A2/G6 + IgG [associated with protection against the blood stages of P. falciparum] vs. 3F11/G10+ ::3E4/H8+ IgG [usually not functionally associated with protection]), we have attempted to estimate the respective proportions of each IgG subtype. In defined culture conditions, Saimiri monkey blood B cells preferentially secrete 3F11/G10+ ::3E4/H8+ IgG in response to parasitized red blood cells. We therefore discuss the conditions that would render this assay suitable for the selection, among P. falciparum blood stage antigens, of those that have major B-cell epitopes. Lymphocytes B de Saimiri sciureus: sécrétion d'IgG dirigés contre les antigènes de Plasmodium falciparum, par des lymphocytes B sensibilisés et restimulés in vitro par des érythrocytes parasités Nous avons isolé, du sang périphérique de Saimiri immuns vis-à-vis des formes sanguines asexuées de Plasmodium falciparum, des lymphocytes B sensibilisés que nous avons restimulés in vitro au moyen d'érythrocytes parasités par P. falciparum et nous avons testé leur aptitude à se différencier en cellules sécrétant des immunoglobulines. Nous avons défini des conditions de culture permettant la détection des IgG sécrétées au moyen d'une technique d'≪immunoblot≫. Les lymphocytes B sensibilisés, restimulés par les globules rouges parasités, se sont révélés sécréter des IgG en présence de lymphocytes T, de monocytes ou de produits de sécrétion lymphocytaires T et monocytaires (cytokines recombinantes humaines). Les lymphocytes B sensibilisés, différenciés, ont sécrété des IgG dirigées contre des molécules des stades sanguins de P. falciparum, comme le montrent des essais d'immunofluorescence indirecte et des analyses en technique de ≪Western blot≫. Comme il est possible, chez Saimiri, de distinguer deux sous-types d'IgG (3F11/G10+ ::3A2/G6+ [associées à la protection contre les formes sanguines de P. falciparum] vs. 3F11/G10+ ::3E4/H8+ [habituellement non associées fonctionnellement à cette protection]), nous avons estimé le niveau des deux sous-types d'IgG. Dans les conditions de culture que nous avons définies, les lymphocytes B de Saimiri sécrètent préférentiellement des IgG de type 3F11/G10+::3E4/H8+ en réponse à une restimulation par les érythrocytes parasités. Nous discutons les conditions dans lesquelles on pourrait inclure cet essai parmi ceux qui permettent de cribler les molécules de P. falciparum contenant des épitopes B protecteurs." @default.
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• W2046333852 date "1993-01-01" @default.
• W2046333852 modified "2023-09-28" @default.
• W2046333852 title "Squirrel monkey (Saimiri sciureus) B lymphocytes: Secretion of IgG directed to Plasmodium falciparum antigens, by primed blood B lymphocytes restimulated in vitro with parasitized red blood cells" @default.
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• W2046333852 doi "https://doi.org/10.1016/0923-2494(93)80124-h" @default.
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