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- W2046333866 abstract "Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m −2 of UV‐C (primarily 254 nm) radiation. but not on photoproducts produced by UV‐B (290–320 nm) radiation in the presence of acetophenone and a N 2 atmosphere or by UV‐A (320–400 nm) radiation in the presence of 4′‐methoxy‐methyltrioxsalen in a N 2 atmosphere and not on the products of OsO 4 oxidation of the DNA. Using end‐labeled DNA of defined sequence, it was possible to identify sites in UV‐C‐irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines." @default.
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- W2046333866 date "1993-03-01" @default.
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- W2046333866 title "Endonuclease activity from tobacco nuclei specific for ultraviolet radiation‐damaged DNA" @default.
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- W2046333866 doi "https://doi.org/10.1111/j.1399-3054.1993.tb01750.x" @default.
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