FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046338579> ?p ?o ?g. }

• W2046338579 endingPage "57" @default.
• W2046338579 startingPage "51" @default.
• W2046338579 abstract "The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me2SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these factors was evaluated comparing post-thawing viability with fluorescent staining (Hoechst bisbenzimide 33258) and the spermatozoa head morphometry, determined with computer-assisted morphology analysis (ASMA). The 1:5 (v/v) dilution ratio resulted in a lower viability in comparison with 1:1 and 1:2 (52.8 ± 2.3% vs. 67.4 ± 2.3% and 65.1 ± 2.3%, respectively, p = 0.0001), but without effects on the head morphology. Although the viability was not significantly different between Me2SO and MeOH (60.4 ± 1.9 vs. 63.2 ± 1.9%, respectively, p = 0.305), a decrease of spermatozoa head area and perimeter was found when spermatozoa were frozen with methanol (6.19 ± 0.01 vs. 6.36 ± 0.01 μm2 and 17.28 ± 0.05 vs. 17.49 ± 0.05 μm, for area and perimeter and MeOH and Me2SO, respectively, p = 0.0001). Finally, a higher viability (75.1 ± 1.7 vs. 48.5 ± 1.7, with or without FBS, respectively, p = 0.0001) and higher spermatozoa head size (6.40 ± 0.01 vs. 6.15 ± 0.01 μm2 and 17.88 ± 0.05 vs. 16.89 ± 0.05 μm, for area and perimeter, with or without FBS, respectively, p = 0.0001) were found when cells were frozen-thawed in freezing media supplemented with FBS. Based on the above findings, dilution ratios lower than 1:5 (v/v) and the addition of serum improved the viability results after cryopreservation. Future studies are required in order to understand the spermatozoa membrane interchange mechanisms in response to the changes in spermatozoa head size caused by cryoprotectants and freezing media supplements." @default.
• W2046338579 created "2016-06-24" @default.
• W2046338579 creator A5016160104 @default.
• W2046338579 creator A5037209861 @default.
• W2046338579 creator A5041051156 @default.
• W2046338579 creator A5046214359 @default.
• W2046338579 creator A5071149509 @default.
• W2046338579 creator A5075182649 @default.
• W2046338579 creator A5084818376 @default.
• W2046338579 creator A5087724235 @default.
• W2046338579 date "2006-08-01" @default.
• W2046338579 modified "2023-10-15" @default.
• W2046338579 title "Cryopreservation of European eel (Anguilla anguilla) spermatozoa: Effect of dilution ratio, foetal bovine serum supplementation, and cryoprotectants" @default.
• W2046338579 cites W1946216546 @default.
• W2046338579 cites W1964868864 @default.
• W2046338579 cites W1964961756 @default.
• W2046338579 cites W1968071540 @default.
• W2046338579 cites W1971812816 @default.
• W2046338579 cites W1980824660 @default.
• W2046338579 cites W1985351380 @default.
• W2046338579 cites W1987445901 @default.
• W2046338579 cites W1990689802 @default.
• W2046338579 cites W1992668797 @default.
• W2046338579 cites W1993309346 @default.
• W2046338579 cites W1996116013 @default.
• W2046338579 cites W1999642866 @default.
• W2046338579 cites W2002093917 @default.
• W2046338579 cites W2015020671 @default.
• W2046338579 cites W2027325261 @default.
• W2046338579 cites W2029021358 @default.
• W2046338579 cites W2031168029 @default.
• W2046338579 cites W2045346520 @default.
• W2046338579 cites W2055535465 @default.
• W2046338579 cites W2062312902 @default.
• W2046338579 cites W2063495390 @default.
• W2046338579 cites W2063894515 @default.
• W2046338579 cites W2064222520 @default.
• W2046338579 cites W2064533140 @default.
• W2046338579 cites W2065694228 @default.
• W2046338579 cites W2068776714 @default.
• W2046338579 cites W2072184522 @default.
• W2046338579 cites W2073583094 @default.
• W2046338579 cites W2074846373 @default.
• W2046338579 cites W2077168419 @default.
• W2046338579 cites W2079220797 @default.
• W2046338579 cites W2085447350 @default.
• W2046338579 cites W2090839433 @default.
• W2046338579 cites W2096233998 @default.
• W2046338579 cites W2111670341 @default.
• W2046338579 cites W2116780002 @default.
• W2046338579 cites W2128102061 @default.
• W2046338579 cites W2132658128 @default.
• W2046338579 cites W2138522540 @default.
• W2046338579 cites W2150603187 @default.
• W2046338579 doi "https://doi.org/10.1016/j.cryobiol.2006.03.011" @default.
• W2046338579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16712830" @default.
• W2046338579 hasPublicationYear "2006" @default.
• W2046338579 type Work @default.
• W2046338579 sameAs 2046338579 @default.
• W2046338579 citedByCount "53" @default.
• W2046338579 countsByYear W20463385792012 @default.
• W2046338579 countsByYear W20463385792013 @default.
• W2046338579 countsByYear W20463385792014 @default.
• W2046338579 countsByYear W20463385792015 @default.
• W2046338579 countsByYear W20463385792016 @default.
• W2046338579 countsByYear W20463385792017 @default.
• W2046338579 countsByYear W20463385792018 @default.
• W2046338579 countsByYear W20463385792019 @default.
• W2046338579 countsByYear W20463385792020 @default.
• W2046338579 countsByYear W20463385792021 @default.
• W2046338579 countsByYear W20463385792022 @default.
• W2046338579 countsByYear W20463385792023 @default.
• W2046338579 crossrefType "journal-article" @default.
• W2046338579 hasAuthorship W2046338579A5016160104 @default.
• W2046338579 hasAuthorship W2046338579A5037209861 @default.
• W2046338579 hasAuthorship W2046338579A5041051156 @default.
• W2046338579 hasAuthorship W2046338579A5046214359 @default.
• W2046338579 hasAuthorship W2046338579A5071149509 @default.
• W2046338579 hasAuthorship W2046338579A5075182649 @default.
• W2046338579 hasAuthorship W2046338579A5084818376 @default.
• W2046338579 hasAuthorship W2046338579A5087724235 @default.
• W2046338579 hasConcept C105702510 @default.
• W2046338579 hasConcept C121332964 @default.
• W2046338579 hasConcept C140793950 @default.
• W2046338579 hasConcept C16685009 @default.
• W2046338579 hasConcept C179933525 @default.
• W2046338579 hasConcept C185592680 @default.
• W2046338579 hasConcept C196843134 @default.
• W2046338579 hasConcept C2524010 @default.
• W2046338579 hasConcept C2778093475 @default.
• W2046338579 hasConcept C2781087480 @default.
• W2046338579 hasConcept C33923547 @default.
• W2046338579 hasConcept C505870484 @default.
• W2046338579 hasConcept C54355233 @default.
• W2046338579 hasConcept C68528507 @default.
• W2046338579 hasConcept C71924100 @default.
• W2046338579 hasConcept C74864618 @default.
• W2046338579 hasConcept C86803240 @default.

• next