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- W2046352904 abstract "The capsular polysaccharide (CPS) of Vibrio cholerae O139 synonym Bengal, which is thought to carry determinants of O-specificity, was isolated by phenol/water extraction followed by delipidation of the contaminating lipopolysaccharide at pH 4.2 and gel-permeation chromatography. The CPS contained d-galactose, 3,6-dideoxy-l-xylo-hexose (colitose, Col), 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2,6-dideoxy-d-glucose (N-acetyl-d-quinovosamine, d-QuiNAc),d-galacturonic acid (d-GalA), and phosphate. The CPS was studied by NMR spectroscopy, methylation analysis, and selective degradations, including partial acid hydrolysis at pH 3.1 and dephosphorylation with aqueous 48% hydrofluoric acid, which both resulted in complete cleavage of Col. It was concluded that the CPS is built up of hexasaccharide repeating units containing inter aliad-galactose 4,6-cyclophosphate and having the following structure These data basically confirm the structure of the V. cholerae CPS proposed on the basis of an NMR study [L. M. Preston et al. (1995) J. Bacteriol. 177, 835–838] and specify exactly the absolute configurations of the constituent monosaccharides and the position of the cyclic phosphate." @default.
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- W2046352904 date "1995-09-01" @default.
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- W2046352904 title "Structure of the Capsular Polysaccharide of Vibrio cholerae O139 Synonym Bengal Containing D-galactose 4,6-Cyclophosphate" @default.
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- W2046352904 doi "https://doi.org/10.1111/j.1432-1033.1995.391zz.x" @default.
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