FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046363258> ?p ?o ?g. }

• W2046363258 endingPage "13353" @default.
• W2046363258 startingPage "13346" @default.
• W2046363258 abstract "N5-Carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) converts 5-aminoimidazole ribonucleotide (AIR), MgATP, and bicarbonate into N5-CAIR, MgADP, and Pi. The enzyme is required for de novo purine biosynthesis in microbes yet is not found in humans suggesting that it represents an ideal and unexplored target for antimicrobial drug design. Here we report the X-ray structures of N5-CAIR synthetase from Escherichia coli with either MgATP or MgADP/Pi bound in the active site cleft. These structures, determined to 1.6-Å resolution, provide detailed information regarding the active site geometry before and after ATP hydrolysis. In both structures, two magnesium ions are observed. Each of these is octahedrally coordinated, and the carboxylate side chain of Glu238 bridges them. For the structure of the MgADP/Pi complex, crystals were grown in the presence of AIR and MgATP. No electron density was observed for AIR, and the electron density corresponding to the nucleotide clearly revealed the presence of ADP and Pi rather than ATP. The bound Pi shifts by approximately 3 Å relative to the γ-phosphoryl group of ATP and forms electrostatic interactions with the side chains of Arg242 and His244. Since the reaction mechanism of N5-CAIR synthetase is believed to proceed via a carboxyphosphate intermediate, we propose that the location of the inorganic phosphate represents the binding site for stabilization of this reactive species. Using the information derived from the two structures reported here, coupled with molecular modeling, we propose a catalytic mechanism for N5-CAIR synthetase." @default.
• W2046363258 created "2016-06-24" @default.
• W2046363258 creator A5011814024 @default.
• W2046363258 creator A5049401708 @default.
• W2046363258 creator A5084141962 @default.
• W2046363258 date "2008-11-20" @default.
• W2046363258 modified "2023-09-24" @default.
• W2046363258 title "Structural Analysis of the Active Site Geometry of <i>N</i>⁵-Carboxyaminoimidazole Ribonucleotide Synthetase from <i>Escherichia coli</i>^," @default.
• W2046363258 cites W1504080251 @default.
• W2046363258 cites W1539796472 @default.
• W2046363258 cites W1824218087 @default.
• W2046363258 cites W1902292659 @default.
• W2046363258 cites W1984039745 @default.
• W2046363258 cites W1987489206 @default.
• W2046363258 cites W1992531977 @default.
• W2046363258 cites W2007857508 @default.
• W2046363258 cites W2020362791 @default.
• W2046363258 cites W2022302833 @default.
• W2046363258 cites W2031975748 @default.
• W2046363258 cites W2032582153 @default.
• W2046363258 cites W2035156848 @default.
• W2046363258 cites W2057984729 @default.
• W2046363258 cites W2065362994 @default.
• W2046363258 cites W2065954942 @default.
• W2046363258 cites W2068412045 @default.
• W2046363258 cites W2068464412 @default.
• W2046363258 cites W2085972597 @default.
• W2046363258 cites W2090771198 @default.
• W2046363258 cites W2093815618 @default.
• W2046363258 cites W2107963900 @default.
• W2046363258 cites W2123859101 @default.
• W2046363258 cites W2126687190 @default.
• W2046363258 cites W2163341755 @default.
• W2046363258 cites W2170031429 @default.
• W2046363258 doi "https://doi.org/10.1021/bi801734z" @default.
• W2046363258 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2677550" @default.
• W2046363258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19053251" @default.
• W2046363258 hasPublicationYear "2008" @default.
• W2046363258 type Work @default.
• W2046363258 sameAs 2046363258 @default.
• W2046363258 citedByCount "27" @default.
• W2046363258 countsByYear W20463632582012 @default.
• W2046363258 countsByYear W20463632582013 @default.
• W2046363258 countsByYear W20463632582014 @default.
• W2046363258 countsByYear W20463632582015 @default.
• W2046363258 countsByYear W20463632582017 @default.
• W2046363258 countsByYear W20463632582018 @default.
• W2046363258 countsByYear W20463632582021 @default.
• W2046363258 countsByYear W20463632582022 @default.
• W2046363258 crossrefType "journal-article" @default.
• W2046363258 hasAuthorship W2046363258A5011814024 @default.
• W2046363258 hasAuthorship W2046363258A5049401708 @default.
• W2046363258 hasAuthorship W2046363258A5084141962 @default.
• W2046363258 hasBestOaLocation W20463632582 @default.
• W2046363258 hasConcept C104317684 @default.
• W2046363258 hasConcept C105768512 @default.
• W2046363258 hasConcept C141315368 @default.
• W2046363258 hasConcept C161790260 @default.
• W2046363258 hasConcept C181199279 @default.
• W2046363258 hasConcept C185592680 @default.
• W2046363258 hasConcept C23265538 @default.
• W2046363258 hasConcept C41183919 @default.
• W2046363258 hasConcept C512185932 @default.
• W2046363258 hasConcept C547475151 @default.
• W2046363258 hasConcept C55493867 @default.
• W2046363258 hasConcept C71240020 @default.
• W2046363258 hasConcept C8010536 @default.
• W2046363258 hasConcept C98892162 @default.
• W2046363258 hasConceptScore W2046363258C104317684 @default.
• W2046363258 hasConceptScore W2046363258C105768512 @default.
• W2046363258 hasConceptScore W2046363258C141315368 @default.
• W2046363258 hasConceptScore W2046363258C161790260 @default.
• W2046363258 hasConceptScore W2046363258C181199279 @default.
• W2046363258 hasConceptScore W2046363258C185592680 @default.
• W2046363258 hasConceptScore W2046363258C23265538 @default.
• W2046363258 hasConceptScore W2046363258C41183919 @default.
• W2046363258 hasConceptScore W2046363258C512185932 @default.
• W2046363258 hasConceptScore W2046363258C547475151 @default.
• W2046363258 hasConceptScore W2046363258C55493867 @default.
• W2046363258 hasConceptScore W2046363258C71240020 @default.
• W2046363258 hasConceptScore W2046363258C8010536 @default.
• W2046363258 hasConceptScore W2046363258C98892162 @default.
• W2046363258 hasIssue "50" @default.
• W2046363258 hasLocation W20463632581 @default.
• W2046363258 hasLocation W20463632582 @default.
• W2046363258 hasLocation W20463632583 @default.
• W2046363258 hasLocation W20463632584 @default.
• W2046363258 hasOpenAccess W2046363258 @default.
• W2046363258 hasPrimaryLocation W20463632581 @default.
• W2046363258 hasRelatedWork W1570438659 @default.
• W2046363258 hasRelatedWork W1996788259 @default.
• W2046363258 hasRelatedWork W2012827119 @default.
• W2046363258 hasRelatedWork W2039437333 @default.
• W2046363258 hasRelatedWork W2044143702 @default.
• W2046363258 hasRelatedWork W2046363258 @default.
• W2046363258 hasRelatedWork W2101174691 @default.
• W2046363258 hasRelatedWork W2134807208 @default.
• W2046363258 hasRelatedWork W2143028632 @default.

• next