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- W2046409379 abstract "Sucrose density gradient analysis of pea and rye leaf homogenates showed that transketolase activity was only present in the supernatant and in the intact chloroplast fraction. It was estimated that in the intact tissue transketolase was predominantly (at least 90%) associated with the chloroplasts. Transketolase was also contained in the proplastids of dark-grown pea roots. By chromatography on DEAE-Sepharose Cl-6B, ammonium sulfate-gradient solubilization, polyacrylamide gel electrophoresis, or isoelectric focusing it was not possible to separate any multiple forms of transketolase from cell-free leaf extracts of rye, wheat, pea, or spinach. Transketolase was also present in the plastids of 70S ribosome-deficient heat-bleached rye leaves, indicating that the chloroplast-localized enzyme was synthesized on cytoplasmic 80S ribosomes." @default.
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- W2046409379 date "1983-05-01" @default.
- W2046409379 modified "2023-09-27" @default.
- W2046409379 title "Plant Transketolase: Subcellular Distribution, Search for Multiple Forms, Site of Synthesis" @default.
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- W2046409379 doi "https://doi.org/10.1016/s0044-328x(83)80107-x" @default.
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