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- W2046453727 abstract "Abstract DTNB has been found to react quantitatively with sulfite ion liberating two moles of 3-carboxylato-4-nitro-thiophenolate ion. While this reaction provides a quantitative measurement of sulfite ion, it also provides quantitative interference with the determination of sulfhydryl groups in proteins. Thiosulfate also interferes with the sulfhydryl determinations using DTNB and the equilibrium constant for the reaction formulated as Eq. (3) is (4.8 ± 0.2) × 10 −2 at pH 7.75 and at ionic strength of 0.4." @default.
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- W2046453727 date "1974-02-01" @default.
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- W2046453727 title "Reactions of thiosulfate and sulfite ions with DTNB: Interference in sulfhydryl group analysis" @default.
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- W2046453727 doi "https://doi.org/10.1016/0003-2697(74)90098-0" @default.
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