FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046453977> ?p ?o ?g. }

• W2046453977 endingPage "11492" @default.
• W2046453977 startingPage "11487" @default.
• W2046453977 abstract "Stable mammalian cell lines harboring a synthetic bovine opsin gene have been derived from the suspension-adapted HEK293 cell line. The opsin gene is under the control of the immediate-early cytomegalovirus promoter/enhancer in an expression vector that also contains a selectable marker (Neo) governed by a relatively weak promoter. The cell lines expressing the opsin gene at high levels are selected by growth in the presence of high concentrations of the antibiotic geneticin. Under the conditions used for cell growth in suspension, opsin is produced at saturated culture levels of more than 2 mg/liter. After reconstitution with 11-cis-retinal, rhodopsin is purified to homogeneity in a single step by immunoaffinity column chromatography. Rhodopsin thus prepared (> 90% recovery at concentrations of up to 15 microM) is indistinguishable from rhodopsin purified from bovine rod outer segments by the following criteria: (i) UV/Vis absorption spectra in the dark and after photobleaching and the rate of metarhodopsin II decay, (ii) initial rates of transducin activation, and (iii) the rate of phosphorylation by rhodopsin kinase. Although mammalian cell opsin migrates slower than rod outer segment opsin on SDS/polyacrylamide gels, presumably due to a different N-glycosylation pattern, their mobilities after deglycosylation are identical. This method has enabled the preparation of several site-specific mutants of bovine opsin in comparable amounts." @default.
• W2046453977 created "2016-06-24" @default.
• W2046453977 creator A5016516519 @default.
• W2046453977 creator A5038162907 @default.
• W2046453977 creator A5053042960 @default.
• W2046453977 date "1996-10-15" @default.
• W2046453977 modified "2023-10-16" @default.
• W2046453977 title "Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines." @default.
• W2046453977 cites W1014800282 @default.
• W2046453977 cites W1579091371 @default.
• W2046453977 cites W1590595627 @default.
• W2046453977 cites W1954929088 @default.
• W2046453977 cites W1969489179 @default.
• W2046453977 cites W1970957929 @default.
• W2046453977 cites W1977062927 @default.
• W2046453977 cites W1989809710 @default.
• W2046453977 cites W1994019984 @default.
• W2046453977 cites W1999851893 @default.
• W2046453977 cites W2014283588 @default.
• W2046453977 cites W2016542329 @default.
• W2046453977 cites W2018289835 @default.
• W2046453977 cites W2048206659 @default.
• W2046453977 cites W2059441263 @default.
• W2046453977 cites W2071451065 @default.
• W2046453977 cites W2083861818 @default.
• W2046453977 cites W2092926963 @default.
• W2046453977 cites W2094967199 @default.
• W2046453977 cites W2100837269 @default.
• W2046453977 cites W2103208001 @default.
• W2046453977 cites W2128635872 @default.
• W2046453977 cites W2337781405 @default.
• W2046453977 cites W70976387 @default.
• W2046453977 doi "https://doi.org/10.1073/pnas.93.21.11487" @default.
• W2046453977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/38084" @default.
• W2046453977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8876162" @default.
• W2046453977 hasPublicationYear "1996" @default.
• W2046453977 type Work @default.
• W2046453977 sameAs 2046453977 @default.
• W2046453977 citedByCount "99" @default.
• W2046453977 countsByYear W20464539772012 @default.
• W2046453977 countsByYear W20464539772013 @default.
• W2046453977 countsByYear W20464539772014 @default.
• W2046453977 countsByYear W20464539772015 @default.
• W2046453977 countsByYear W20464539772016 @default.
• W2046453977 countsByYear W20464539772017 @default.
• W2046453977 countsByYear W20464539772018 @default.
• W2046453977 countsByYear W20464539772020 @default.
• W2046453977 countsByYear W20464539772021 @default.
• W2046453977 countsByYear W20464539772022 @default.
• W2046453977 countsByYear W20464539772023 @default.
• W2046453977 crossrefType "journal-article" @default.
• W2046453977 hasAuthorship W2046453977A5016516519 @default.
• W2046453977 hasAuthorship W2046453977A5038162907 @default.
• W2046453977 hasAuthorship W2046453977A5053042960 @default.
• W2046453977 hasBestOaLocation W20464539771 @default.
• W2046453977 hasConcept C104317684 @default.
• W2046453977 hasConcept C131667965 @default.
• W2046453977 hasConcept C143065580 @default.
• W2046453977 hasConcept C149574991 @default.
• W2046453977 hasConcept C153911025 @default.
• W2046453977 hasConcept C202033177 @default.
• W2046453977 hasConcept C2780827179 @default.
• W2046453977 hasConcept C30028475 @default.
• W2046453977 hasConcept C54355233 @default.
• W2046453977 hasConcept C55493867 @default.
• W2046453977 hasConcept C81885089 @default.
• W2046453977 hasConcept C86803240 @default.
• W2046453977 hasConceptScore W2046453977C104317684 @default.
• W2046453977 hasConceptScore W2046453977C131667965 @default.
• W2046453977 hasConceptScore W2046453977C143065580 @default.
• W2046453977 hasConceptScore W2046453977C149574991 @default.
• W2046453977 hasConceptScore W2046453977C153911025 @default.
• W2046453977 hasConceptScore W2046453977C202033177 @default.
• W2046453977 hasConceptScore W2046453977C2780827179 @default.
• W2046453977 hasConceptScore W2046453977C30028475 @default.
• W2046453977 hasConceptScore W2046453977C54355233 @default.
• W2046453977 hasConceptScore W2046453977C55493867 @default.
• W2046453977 hasConceptScore W2046453977C81885089 @default.
• W2046453977 hasConceptScore W2046453977C86803240 @default.
• W2046453977 hasIssue "21" @default.
• W2046453977 hasLocation W20464539771 @default.
• W2046453977 hasLocation W20464539772 @default.
• W2046453977 hasLocation W20464539773 @default.
• W2046453977 hasLocation W20464539774 @default.
• W2046453977 hasOpenAccess W2046453977 @default.
• W2046453977 hasPrimaryLocation W20464539771 @default.
• W2046453977 hasRelatedWork W157460415 @default.
• W2046453977 hasRelatedWork W1603853902 @default.
• W2046453977 hasRelatedWork W1965340846 @default.
• W2046453977 hasRelatedWork W1986668976 @default.
• W2046453977 hasRelatedWork W1995934066 @default.
• W2046453977 hasRelatedWork W2001526908 @default.
• W2046453977 hasRelatedWork W2001820714 @default.
• W2046453977 hasRelatedWork W2002714238 @default.
• W2046453977 hasRelatedWork W2016100843 @default.
• W2046453977 hasRelatedWork W2072669759 @default.
• W2046453977 hasVolume "93" @default.
• W2046453977 isParatext "false" @default.

• next