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- W2046457549 abstract "We expressed a truncated p1 protein (p1-379) from the Saccharomyces cerevisiae retrotransposon Ty1 in the cytosol of Escherichia coli and Pichia pastoris, achieving maximum expression levels of 20 and 65 mg/l, respectively. Two well-characterized epitopes from beet necrotic yellow vein virus (BNYVV) were used to evaluate the virus-like particles (VLPs) as a presentation system for synthetic antigens. The epitopes were placed near the externally located N-terminus and at the internally located C-terminus of the p1 protein. Electron microscopy showed all particles to be morphologically similar to wild-type Ty1-VLPs from S. cerevisiae. However, fewer VLPs were observed in P. pastoris, suggesting that posttranslational modifications might inhibit binding to the carbon-coated electron microscopy grids. BNYVV epitopes were detected with specific monoclonal antibodies by Western blot and ELISA, with a detection limit as low as 5 ng/ml. The VLPs are therefore promising candidates for diagnostic standards and future vaccine development." @default.
- W2046457549 created "2016-06-24" @default.
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- W2046457549 date "2010-01-01" @default.
- W2046457549 modified "2023-09-25" @default.
- W2046457549 title "Characterization and Diagnostic Potential of Foreign Epitope-Presenting Ty1 Virus-Like Particles Expressed in <i>Escherichia coli</i> and <i>Pichia pastoris</i>" @default.
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- W2046457549 doi "https://doi.org/10.1159/000274311" @default.
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