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- W204645890 abstract "Melanocortins, such as ACTH, bind to a family of receptors: melanocortin receptors 1 to 5. Melanocortin 3 receptors (MC3-R) expressed in the brain are well characterized however the role of MC3-R in the periphery is unclear. We have recently described immunopositive staining for MC3-R in the testes of wild type mice and reported that the testicular histology of the MC3-R null mouse was abnormal (Wotherspoon et al 2008). The aims of this further work were to confirm that MC3-R is expressed in testes and determine if ACTH affects testicular steroidogenesis in vitro.RNA was extracted from the testes of sexually mature wild type (C57 B1.6) using Trizol (Invitrogen). MMLV-reverse transcriptase and random primers were used to generate cDNA, with no MMLV-RT in the non-template control. Primers for MC3-R were used as previously described (Getting et al 1999). To ensure that MC3-R was also expressed at the protein level, Western blotting of mouse testes lysate was done using a standard protocol with a previously characterized rabbit anti-MC3-R (Leoni et al 2008). In another series of experiments, testes collected from sexually mature wild type mice were hemi-sected and incubated in McCoy’s 5A media ± ACTH1-39 (10-10M) ± LH (2 ng/ml) for 5 hours at 35°C in air. At the end of the incubation the media were removed and frozen until assayed for testosterone by RIA. Each treatment was done in quadruplicate and each experiment was repeated 2 or 3 times. The results are presented as the mean of the means of each experiment ± SEM. *P<0.05 versus appropriate control." @default.
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- W204645890 date "2009-01-01" @default.
- W204645890 modified "2023-09-23" @default.
- W204645890 title "Molecular characterization of the melanocortin 3 receptor in the testes of mice" @default.
- W204645890 hasPublicationYear "2009" @default.
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