Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046505514> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2046505514 endingPage "11832" @default.
- W2046505514 startingPage "11819" @default.
- W2046505514 abstract "The nature and variety in the denatured state of a protein, a non-native state under a given set of conditions, has been a subject of intense debate. Here, using multidimensional NMR, we have characterized the 6 M Gdn-HCl-denatured state of GED, the assembly domain of dynamin. Even under such strongly denaturing conditions, we detected the presence of conformations in slow exchange on the NMR chemical shift time scale. Although the GED oligomer as well as the SDS-denatured monomeric GED were seen to be predominantly helical [Chugh et al. (2006) FEBS J. 273, 388−397], the 6 M Gdn-HCl-denatured GED has largely β-structural preferences. However, against such a background, we could detect the presence of a population with a short helical stretch (Arg42-Ile47) in the ensemble. The 1H−1H NOEs suggested presence of pockets of transient short-range order along the chain. Put together these segments may lead to a rather small number of interconverting topologically distinguishable ensembles. Spectral density analysis of 15N relaxation rates and {1H}−15N NOE, measured at 600 and 800 MHz, and comparison of J(0) with hydrophobic patches calculated using AABUF approach, indicated presence of four domains of slow motions. These coincided to a large extent with those showing significant Rex. Additionally, a proline residue in the connection between two of these domains seems to cause a fast hinge motion. These observations help enhance our understanding of protein denatured states, and of folding concepts, in general." @default.
- W2046505514 created "2016-06-24" @default.
- W2046505514 creator A5048765273 @default.
- W2046505514 creator A5055374689 @default.
- W2046505514 creator A5083086225 @default.
- W2046505514 date "2007-10-01" @default.
- W2046505514 modified "2023-09-25" @default.
- W2046505514 title "Pockets of Short-Range Transient Order and Restricted Topological Heterogeneity in the Guanidine-Denatured State Ensemble of GED of Dynamin" @default.
- W2046505514 cites W1481303389 @default.
- W2046505514 cites W1510998615 @default.
- W2046505514 cites W1563186874 @default.
- W2046505514 cites W1968859256 @default.
- W2046505514 cites W1971919907 @default.
- W2046505514 cites W1984916779 @default.
- W2046505514 cites W1992798240 @default.
- W2046505514 cites W1998807178 @default.
- W2046505514 cites W2003119577 @default.
- W2046505514 cites W2015737242 @default.
- W2046505514 cites W2016170088 @default.
- W2046505514 cites W2021006927 @default.
- W2046505514 cites W2029885033 @default.
- W2046505514 cites W2037021218 @default.
- W2046505514 cites W2045950270 @default.
- W2046505514 cites W2058454436 @default.
- W2046505514 cites W2061793653 @default.
- W2046505514 cites W2062710766 @default.
- W2046505514 cites W2067237119 @default.
- W2046505514 cites W2074070436 @default.
- W2046505514 cites W20868186 @default.
- W2046505514 cites W2086951107 @default.
- W2046505514 cites W2089387771 @default.
- W2046505514 cites W2092818861 @default.
- W2046505514 cites W2099698581 @default.
- W2046505514 cites W2103047799 @default.
- W2046505514 cites W2107165577 @default.
- W2046505514 cites W2145990894 @default.
- W2046505514 cites W2148521301 @default.
- W2046505514 cites W278916191 @default.
- W2046505514 cites W4242009576 @default.
- W2046505514 doi "https://doi.org/10.1021/bi701280p" @default.
- W2046505514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17910478" @default.
- W2046505514 hasPublicationYear "2007" @default.
- W2046505514 type Work @default.
- W2046505514 sameAs 2046505514 @default.
- W2046505514 citedByCount "20" @default.
- W2046505514 countsByYear W20465055142012 @default.
- W2046505514 countsByYear W20465055142017 @default.
- W2046505514 countsByYear W20465055142018 @default.
- W2046505514 crossrefType "journal-article" @default.
- W2046505514 hasAuthorship W2046505514A5048765273 @default.
- W2046505514 hasAuthorship W2046505514A5055374689 @default.
- W2046505514 hasAuthorship W2046505514A5083086225 @default.
- W2046505514 hasConcept C1491633281 @default.
- W2046505514 hasConcept C156407911 @default.
- W2046505514 hasConcept C178790620 @default.
- W2046505514 hasConcept C185592680 @default.
- W2046505514 hasConcept C204328495 @default.
- W2046505514 hasConcept C2779463914 @default.
- W2046505514 hasConcept C2780642029 @default.
- W2046505514 hasConcept C28005876 @default.
- W2046505514 hasConcept C55493867 @default.
- W2046505514 hasConcept C8010536 @default.
- W2046505514 hasConceptScore W2046505514C1491633281 @default.
- W2046505514 hasConceptScore W2046505514C156407911 @default.
- W2046505514 hasConceptScore W2046505514C178790620 @default.
- W2046505514 hasConceptScore W2046505514C185592680 @default.
- W2046505514 hasConceptScore W2046505514C204328495 @default.
- W2046505514 hasConceptScore W2046505514C2779463914 @default.
- W2046505514 hasConceptScore W2046505514C2780642029 @default.
- W2046505514 hasConceptScore W2046505514C28005876 @default.
- W2046505514 hasConceptScore W2046505514C55493867 @default.
- W2046505514 hasConceptScore W2046505514C8010536 @default.
- W2046505514 hasIssue "42" @default.
- W2046505514 hasLocation W20465055141 @default.
- W2046505514 hasLocation W20465055142 @default.
- W2046505514 hasOpenAccess W2046505514 @default.
- W2046505514 hasPrimaryLocation W20465055141 @default.
- W2046505514 hasRelatedWork W2001584154 @default.
- W2046505514 hasRelatedWork W2039222044 @default.
- W2046505514 hasRelatedWork W2045060966 @default.
- W2046505514 hasRelatedWork W2076619122 @default.
- W2046505514 hasRelatedWork W2150609099 @default.
- W2046505514 hasRelatedWork W2341254035 @default.
- W2046505514 hasRelatedWork W2949090308 @default.
- W2046505514 hasRelatedWork W2949979104 @default.
- W2046505514 hasRelatedWork W2951316285 @default.
- W2046505514 hasRelatedWork W4205450956 @default.
- W2046505514 hasVolume "46" @default.
- W2046505514 isParatext "false" @default.
- W2046505514 isRetracted "false" @default.
- W2046505514 magId "2046505514" @default.
- W2046505514 workType "article" @default.