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• W2046543201 abstract "Extra virgin olive oil (EVOO) has demonstrated great anti-inflammatory properties. Nowadays, it is clear that its minor components have a key role in these beneficial effects. However, the contribution of the unsaponifiable fraction (UF) to these healthy effects remains unknown. The present study was designed to evaluate UF in LPS stimulated peritoneal macrophages isolated from mice. NO production was analysed by the Griess method and intracellular ROS by fluorescence analysis. In addition, MAPK family activation, IKBα degradation, NFκB-p65, iNOS and COX-2 expression were determined by Western blot. UF exerted anti-inflammatory and anti-oxidant effects inhibiting LPS-induced intracellular ROS and nitrite production. Additionally, UF decreased COX-2 and iNOS protein expression. These effects were related with a down-regulation in NFκB signal signalling pathways and in MAPK phosphorylation. UF of EVOO compounds could play an important role in the anti-inflammatory effect of virgin olive oils and probably provide an attractive complement in management of inflammatory diseases." @default.
• W2046543201 created "2016-06-24" @default.
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• W2046543201 date "2014-03-01" @default.
• W2046543201 modified "2023-10-18" @default.
• W2046543201 title "Unsaponifiable fraction from extra virgin olive oil inhibits the inflammatory response in LPS-activated murine macrophages" @default.
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• W2046543201 doi "https://doi.org/10.1016/j.foodchem.2013.09.117" @default.
• W2046543201 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24206694" @default.
• W2046543201 hasPublicationYear "2014" @default.
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