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• W2046553616 abstract "We have developed a new concept involving a single-step homogeneous method for single-nucleotide polymorphism (SNP) typing. In this method, a probe containing base-discriminating fluorescent (BDF) bases is added to a sample solution. BDF base-containing DNA usually shows only a weak fluorescence, but emits a strong blue fluorescence when it recognizes a target base at a specific site in a hybridized strand. By utilizing this feature, a simple mix-and-read SNP typing assay was achieved without any tedious probe-designing or washing processes for exclusion of hybridization error or any addition of DNA-modifying enzymes. This is very different from conventional methods. We simultaneously analyzed a number of samples with ease, with a high accuracy, using our BDF assay." @default.
• W2046553616 created "2016-06-24" @default.
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• W2046553616 date "2006-01-01" @default.
• W2046553616 modified "2023-10-14" @default.
• W2046553616 title "Simple SNP typing assay using a base-discriminating fluorescent probe" @default.
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• W2046553616 doi "https://doi.org/10.1039/b515923g" @default.
• W2046553616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16880929" @default.
• W2046553616 hasPublicationYear "2006" @default.
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