FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046554798> ?p ?o ?g. }

• W2046554798 endingPage "3801" @default.
• W2046554798 startingPage "3792" @default.
• W2046554798 abstract "Smooth muscle caldesmon is a single polypeptide chain with its NH2- and COOH-terminal domains separated by a long alpha-helix. Caldesmon was labeled at either Cys-153 in the NH2 domain or Cys-580 in the COOH domain with a variety of fluorescence probes. Fluorescence intensity, peak position, and polarization of probes on Cys-580 were very sensitive to the binding to actin (with or without tropomyosin), whereas for probes on Cys-153, there was a lack of response, in reconstituted or native actin thin filaments. From fluorescence resonance energy transfer from donor labels on either caldesmon cysteine to acceptor labels on Cys-374 of actin, the distance between the donor and acceptor was estimated to be 27 A for the donor at Cys-580 and 65-80 A for the donor at Cys-153. These findings were the same for caldesmon prepared with or without heat treatment and with striated or smooth muscle actin. These results, together with previous knowledge that COOH-terminal fragments of caldesmon bind to actin whereas NH2-terminal fragments do not, indicate that, while the COOH domain of caldesmon is bound to actin, the NH2 domain is largely dissociated. Fluorescence quenching studies showed that actin binding to caldesmon greatly decreased the accessibility of probes at caldesmon Cys-580 to the quencher, whereas for probes at Cys-153, actin afforded much less, but significant, protection from quenching. Consequently, it appears that, although the NH2 domain is mostly dissociated, it spends some time in the vicinity of actin, through either a weak interaction with actin or collisions with actin and/or because of restricted flexibility which constrains the NH2 domain to be close to the actin filament. Since the NH2 domain of caldesmon binds to the neck region of myosin, a dissociated NH2 domain may account for caldesmon's ability to link myosin and actin filaments." @default.
• W2046554798 created "2016-06-24" @default.
• W2046554798 creator A5057469369 @default.
• W2046554798 date "1997-04-01" @default.
• W2046554798 modified "2023-09-24" @default.
• W2046554798 title "Arrangement of the COOH-Terminal and NH₂-Terminal Domains of Caldesmon Bound to Actin" @default.
• W2046554798 cites W1486373758 @default.
• W2046554798 cites W1492452850 @default.
• W2046554798 cites W1505995599 @default.
• W2046554798 cites W1516201963 @default.
• W2046554798 cites W1523556909 @default.
• W2046554798 cites W1532023408 @default.
• W2046554798 cites W1565313767 @default.
• W2046554798 cites W1573771296 @default.
• W2046554798 cites W1577528912 @default.
• W2046554798 cites W1578846125 @default.
• W2046554798 cites W1578984770 @default.
• W2046554798 cites W1580371254 @default.
• W2046554798 cites W1609692701 @default.
• W2046554798 cites W1726148612 @default.
• W2046554798 cites W1832713597 @default.
• W2046554798 cites W1857317048 @default.
• W2046554798 cites W1965337151 @default.
• W2046554798 cites W1969184507 @default.
• W2046554798 cites W1971753650 @default.
• W2046554798 cites W1981127731 @default.
• W2046554798 cites W1988857652 @default.
• W2046554798 cites W1998205802 @default.
• W2046554798 cites W2022666731 @default.
• W2046554798 cites W2026064166 @default.
• W2046554798 cites W2044536426 @default.
• W2046554798 cites W2051056116 @default.
• W2046554798 cites W2052350595 @default.
• W2046554798 cites W2074261984 @default.
• W2046554798 cites W2078134722 @default.
• W2046554798 cites W2082126819 @default.
• W2046554798 cites W2090890789 @default.
• W2046554798 cites W2092981098 @default.
• W2046554798 cites W2131970139 @default.
• W2046554798 cites W2319745991 @default.
• W2046554798 cites W2341269293 @default.
• W2046554798 cites W2384297415 @default.
• W2046554798 cites W4299496276 @default.
• W2046554798 doi "https://doi.org/10.1021/bi961652w" @default.
• W2046554798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9092808" @default.
• W2046554798 hasPublicationYear "1997" @default.
• W2046554798 type Work @default.
• W2046554798 sameAs 2046554798 @default.
• W2046554798 citedByCount "7" @default.
• W2046554798 crossrefType "journal-article" @default.
• W2046554798 hasAuthorship W2046554798A5057469369 @default.
• W2046554798 hasConcept C121332964 @default.
• W2046554798 hasConcept C121745418 @default.
• W2046554798 hasConcept C12554922 @default.
• W2046554798 hasConcept C125705527 @default.
• W2046554798 hasConcept C130861313 @default.
• W2046554798 hasConcept C142669718 @default.
• W2046554798 hasConcept C1491633281 @default.
• W2046554798 hasConcept C181199279 @default.
• W2046554798 hasConcept C185592680 @default.
• W2046554798 hasConcept C205875245 @default.
• W2046554798 hasConcept C2776190535 @default.
• W2046554798 hasConcept C2779201268 @default.
• W2046554798 hasConcept C2779360546 @default.
• W2046554798 hasConcept C29688787 @default.
• W2046554798 hasConcept C2993400109 @default.
• W2046554798 hasConcept C55493867 @default.
• W2046554798 hasConcept C62520636 @default.
• W2046554798 hasConcept C8010536 @default.
• W2046554798 hasConcept C86803240 @default.
• W2046554798 hasConcept C91881484 @default.
• W2046554798 hasConcept C96305047 @default.
• W2046554798 hasConceptScore W2046554798C121332964 @default.
• W2046554798 hasConceptScore W2046554798C121745418 @default.
• W2046554798 hasConceptScore W2046554798C12554922 @default.
• W2046554798 hasConceptScore W2046554798C125705527 @default.
• W2046554798 hasConceptScore W2046554798C130861313 @default.
• W2046554798 hasConceptScore W2046554798C142669718 @default.
• W2046554798 hasConceptScore W2046554798C1491633281 @default.
• W2046554798 hasConceptScore W2046554798C181199279 @default.
• W2046554798 hasConceptScore W2046554798C185592680 @default.
• W2046554798 hasConceptScore W2046554798C205875245 @default.
• W2046554798 hasConceptScore W2046554798C2776190535 @default.
• W2046554798 hasConceptScore W2046554798C2779201268 @default.
• W2046554798 hasConceptScore W2046554798C2779360546 @default.
• W2046554798 hasConceptScore W2046554798C29688787 @default.
• W2046554798 hasConceptScore W2046554798C2993400109 @default.
• W2046554798 hasConceptScore W2046554798C55493867 @default.
• W2046554798 hasConceptScore W2046554798C62520636 @default.
• W2046554798 hasConceptScore W2046554798C8010536 @default.
• W2046554798 hasConceptScore W2046554798C86803240 @default.
• W2046554798 hasConceptScore W2046554798C91881484 @default.
• W2046554798 hasConceptScore W2046554798C96305047 @default.
• W2046554798 hasIssue "13" @default.
• W2046554798 hasLocation W20465547981 @default.
• W2046554798 hasLocation W20465547982 @default.
• W2046554798 hasOpenAccess W2046554798 @default.
• W2046554798 hasPrimaryLocation W20465547981 @default.

• next