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• W2046564897 abstract "Monoclonal antibodies were produced against two structurally related tumor necrosis factors (TNFs), TNF -α (previously called tumor necrosis factor) and TNF-β (previously called lymphotoxin). The potential of these antibodies for the purification of TNFs, the development of specific immunoassays, and for defining the antigenic and functional domains of these cytokines was investigated. None of the monoclonal antibodies cross-reacted with both TNF-α and TNF-β, or reacted with synthetic peptides which represented several of the regions of homology between these cytokines. Neutralizing monoclonal antibodies were utilized as immunoadsorbents to purify recombinant TNF-α and TNF-β from E. coli lysates. TNFs purified by this method were greater than 98 percent pure by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and exhibited specific activities that were the same as TNFs isolated from natural sources using conventional Chromatographic techniques. In addition, specific ELISA assays were developed that could detect less than 1 ng/ml of TNF-α or TNF-β, and in contrast to bioassays, could discriminate between these related cytokines." @default.
• W2046564897 created "2016-06-24" @default.
• W2046564897 creator A5030567899 @default.
• W2046564897 creator A5048675624 @default.
• W2046564897 date "1987-10-01" @default.
• W2046564897 modified "2023-09-25" @default.
• W2046564897 title "Monoclonal Antibodies to Human Tumor Necrosis Factors Alpha and Beta: Application for Affinity Purification, Immunoassays, and as Structural Probes" @default.
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• W2046564897 doi "https://doi.org/10.1089/hyb.1987.6.489" @default.
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