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- W2046587219 abstract "Soybean is one of the main crops grown in Yunnan Province, China, where the crop is grown in spring, summer and autumn. Occurrence of a disease was observed in this region in 2002 with symptoms resembling red crown rot (Russin & Kuruppu, 1999). The top leaves of infected plants had interveinal necrosis, while small red spheres were scattered or concentrated on roots and stems bases, which also had black, streaky lesions. The outer surface of stems from infected plants was dark in colour. In some autumn and summer crops, the disease caused severe yield losses. Plants with symptoms were sampled from fields of autumn-season soybean crops. Red spherical fruiting bodies were isolated from diseased tissues. Microscopic examination revealed that these were perithecia, 310–510 µm in diameter and 325–520 µm in length. Under gentle pressure, asci and ascospores were exuded from these bodies. The asci were curved, fusoid, shuttle-shaped, 10–23 µm in diameter and 101–150 µm in length. Ascospores were rod-shaped or falcate, with mostly rounded ends, double-celled, and 4·5–5·6 µm in diameter and 30–54 µm in length. Isolation was made from stem tissues at the edge of disease lesions on potato dextrose agar (PDA) at 28°C. Hyphae were observed after incubation for 2 days and conidia were seen after 3–4 days. The conidia measured 4·2–6 µm in diameter and 30–65 µm in length, with terminal budding from conidiophores. Conidia were cylindrical and had one or two cells. The fungus was identified as Cylindrocladium crotalariae (teleomorph Calonectria crotalariae). The isolate was grown on PDA and mixed with sterilized soil in pots. Soybean seeds (cv. Dian Cang 2) were planted in the pots and placed in glasshouse at 25 ± 2°C. Leaf and stem symptoms of red crown rot were observed in the plants and C. crotalariae was isolated from these diseased plants, according to Hwang's method (Hwang & Ko, 1976). This is first finding of soybean red crown rot in Yunnan province, where outbreaks of this disease have been observed in counties in the central and eastern parts of the province, and where autumn soybeans are planted after summer rice. This pathogen has been previously reported in China in Jiangsu Province (Gai, 1992) but is not known elsewhere." @default.
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- W2046587219 date "2004-08-01" @default.
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- W2046587219 title "Cylindrocladium crotalariae causing red crown rot of soybean in China" @default.
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- W2046587219 doi "https://doi.org/10.1111/j.1365-3059.2004.01036.x" @default.
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