FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046594654> ?p ?o ?g. }

• W2046594654 endingPage "403" @default.
• W2046594654 startingPage "394" @default.
• W2046594654 abstract "Specific interactions of growth factors with heparan sulfate may function as switches to regulate stages of branching morphogenesis in developing mammalian organs, such as breast, lung, salivary gland and kidney, but the evidence derives mostly from studies of explanted tissues or cell culture (Shah et al., 2004). We recently provided in vivo evidence that inactivation of Ndst1, the predominant N-deacetylase/N-sulfotransferase gene essential for the formation of mature heparan sulfate, results in a highly specific defect in murine lobuloalveolar development (Crawford et al., 2010). Here, we demonstrate a highly penetrant dramatic defect in primary branching by mammary epithelial-specific inactivation of Ext1, a subunit of the copolymerase complex that catalyzes the formation of the heparan sulfate chain. In contrast to Ext1 deletion, inactivation of Hs2st (which encodes an enzyme required for 2-O-sulfation of uronic acids in heparan sulfate) did not inhibit ductal formation but displayed markedly decreased secondary and ductal side-branches as well as fewer bifurcated terminal end buds. Targeted conditional deletion of c-Met, the receptor for HGF, in mammary epithelial cells showed similar defects in secondary and ductal side-branching, but did not result in any apparent defect in bifurcation of terminal end buds. Although there is published evidence indicating a role for 2-O sulfation in HGF binding, primary epithelial cells isolated from Hs2st conditional deletions were able to activate Erk in the presence of HGF and there appeared to be only a slight reduction in HGF-mediated c-Met phosphorylation in these cells compared to control. Thus, both c-Met and Hs2st play important, but partly independent, roles in secondary and ductal side-branching. When considered together with previous studies of Ndst1-deficient glands, the data presented here raise the possibility of partially-independent regulation by heparan sulfate-dependent pathways of primary ductal branching, terminal end bud bifurcation, secondary branching, ductal side-branching and lobuloalveolar formation." @default.
• W2046594654 created "2016-06-24" @default.
• W2046594654 creator A5022684460 @default.
• W2046594654 creator A5031825923 @default.
• W2046594654 creator A5044617581 @default.
• W2046594654 creator A5053570913 @default.
• W2046594654 creator A5059137254 @default.
• W2046594654 creator A5061745258 @default.
• W2046594654 creator A5067976238 @default.
• W2046594654 date "2011-07-01" @default.
• W2046594654 modified "2023-10-15" @default.
• W2046594654 title "Stage-dependent regulation of mammary ductal branching by heparan sulfate and HGF-cMet signaling" @default.
• W2046594654 cites W1217425532 @default.
• W2046594654 cites W1523335221 @default.
• W2046594654 cites W1597685410 @default.
• W2046594654 cites W1971351550 @default.
• W2046594654 cites W1977923248 @default.
• W2046594654 cites W1978514461 @default.
• W2046594654 cites W1981098429 @default.
• W2046594654 cites W1984294894 @default.
• W2046594654 cites W1985218254 @default.
• W2046594654 cites W1985995289 @default.
• W2046594654 cites W1996795546 @default.
• W2046594654 cites W1998479997 @default.
• W2046594654 cites W2006641366 @default.
• W2046594654 cites W2010040980 @default.
• W2046594654 cites W2013954497 @default.
• W2046594654 cites W2025212378 @default.
• W2046594654 cites W2034887388 @default.
• W2046594654 cites W2038425754 @default.
• W2046594654 cites W2042619286 @default.
• W2046594654 cites W2043484559 @default.
• W2046594654 cites W2043931120 @default.
• W2046594654 cites W2046368360 @default.
• W2046594654 cites W2046616497 @default.
• W2046594654 cites W2049056185 @default.
• W2046594654 cites W2051399767 @default.
• W2046594654 cites W2054445281 @default.
• W2046594654 cites W2056113774 @default.
• W2046594654 cites W2057179778 @default.
• W2046594654 cites W2062128415 @default.
• W2046594654 cites W2065695059 @default.
• W2046594654 cites W2067188105 @default.
• W2046594654 cites W2067314001 @default.
• W2046594654 cites W2068575434 @default.
• W2046594654 cites W2074415567 @default.
• W2046594654 cites W2077664347 @default.
• W2046594654 cites W2080069562 @default.
• W2046594654 cites W2080356314 @default.
• W2046594654 cites W2082133069 @default.
• W2046594654 cites W2082251348 @default.
• W2046594654 cites W2082541916 @default.
• W2046594654 cites W2090274013 @default.
• W2046594654 cites W2098294628 @default.
• W2046594654 cites W2098619103 @default.
• W2046594654 cites W2099134569 @default.
• W2046594654 cites W2099851642 @default.
• W2046594654 cites W2100401891 @default.
• W2046594654 cites W2102979193 @default.
• W2046594654 cites W2110278880 @default.
• W2046594654 cites W2110715199 @default.
• W2046594654 cites W2116715149 @default.
• W2046594654 cites W2117282567 @default.
• W2046594654 cites W2121136307 @default.
• W2046594654 cites W2123217377 @default.
• W2046594654 cites W2125279675 @default.
• W2046594654 cites W2128584428 @default.
• W2046594654 cites W2128739635 @default.
• W2046594654 cites W2129115386 @default.
• W2046594654 cites W2130642820 @default.
• W2046594654 cites W2132493358 @default.
• W2046594654 cites W2140861834 @default.
• W2046594654 cites W2141025340 @default.
• W2046594654 cites W2141843510 @default.
• W2046594654 cites W2143380875 @default.
• W2046594654 cites W2143633028 @default.
• W2046594654 cites W2147927776 @default.
• W2046594654 cites W2149120477 @default.
• W2046594654 cites W2149488419 @default.
• W2046594654 cites W2155687693 @default.
• W2046594654 cites W2156014566 @default.
• W2046594654 cites W2159141617 @default.
• W2046594654 cites W2159615789 @default.
• W2046594654 cites W2161742564 @default.
• W2046594654 cites W2164205795 @default.
• W2046594654 cites W2169829977 @default.
• W2046594654 cites W2170361206 @default.
• W2046594654 cites W2183437149 @default.
• W2046594654 cites W2293671377 @default.
• W2046594654 cites W2333269758 @default.
• W2046594654 cites W2407636133 @default.
• W2046594654 cites W2410702549 @default.
• W2046594654 cites W4238813159 @default.
• W2046594654 cites W4242945710 @default.
• W2046594654 doi "https://doi.org/10.1016/j.ydbio.2011.04.035" @default.
• W2046594654 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3118867" @default.
• W2046594654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21586278" @default.
• W2046594654 hasPublicationYear "2011" @default.

• next