FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046643103> ?p ?o ?g. }

• W2046643103 endingPage "55" @default.
• W2046643103 startingPage "46" @default.
• W2046643103 abstract "The invertebrate glutamate-gated chloride channels (GluCls) are receptor molecules and targets for the avermectin-milbemycin (AM) group of anthelmintics. Mutations in GluCls are associated with ivermectin resistance in the soil dwelling nematode Caenorhabditis elegans and the parasitic nematode Cooperia oncophora. In this study, full-length cDNAs encoding alpha and beta subunits of GluCl were cloned and sequenced in Cylicocyclus nassatus, a common and important cyathostomin nematode parasite of horses. Both genes possess the sequence characteristics typical of GluCls, and phylogenetic analysis confirms that these genes are evolutionarily closely related to GluCls of other nematodes and flies. Complete coding sequences of C. nassatus GluCl-alpha and GluCl-beta were subcloned into pTL1 mammalian expression vector, and proteins were expressed in COS-7 cells. Ivermectin-binding characteristics were determined by incubating COS-7 cell membranes expressing C. nassatus GluCl-alpha and GluCl-beta proteins with [(3)H]ivermectin. In competitive binding experiments, fitting the data to a one site competition model, C. nassatus GluCl-alpha was found to bind [(3)H]ivermectin with a high amount of displaceable binding (IC(50)=208 pM). Compared to the mock-transfected COS-7 cells, the means of [(3)H]ivermectin binding were significantly different for C. nassatus GluCl-alpha and the Haemonchus contortus GluCl (HcGluCla) (p=0.018 and 0.023, respectively) but not for C. nassatus GluCl-beta (p=0.370). This is the first report of orthologs of GluCl genes and in vitro expression of an ivermectin-binding protein in a cyathostomin species. These data suggest the likelihood of a similar mechanism of action of AM drugs in these parasites, and suggest that mechanisms of resistance may also be similar." @default.
• W2046643103 created "2016-06-24" @default.
• W2046643103 creator A5028707814 @default.
• W2046643103 creator A5056170287 @default.
• W2046643103 creator A5085984380 @default.
• W2046643103 date "2006-11-01" @default.
• W2046643103 modified "2023-09-26" @default.
• W2046643103 title "Cloning and characterization of genes encoding α and β subunits of glutamate-gated chloride channel protein in Cylicocyclus nassatus" @default.
• W2046643103 cites W132231329 @default.
• W2046643103 cites W1552971676 @default.
• W2046643103 cites W174745564 @default.
• W2046643103 cites W1971446667 @default.
• W2046643103 cites W1976054510 @default.
• W2046643103 cites W1981722489 @default.
• W2046643103 cites W1985784698 @default.
• W2046643103 cites W1989706787 @default.
• W2046643103 cites W2009996427 @default.
• W2046643103 cites W2013305180 @default.
• W2046643103 cites W2015354156 @default.
• W2046643103 cites W2034032394 @default.
• W2046643103 cites W2039711528 @default.
• W2046643103 cites W2040901343 @default.
• W2046643103 cites W2049519417 @default.
• W2046643103 cites W2049914659 @default.
• W2046643103 cites W2052109803 @default.
• W2046643103 cites W2052600754 @default.
• W2046643103 cites W2056777384 @default.
• W2046643103 cites W2057264061 @default.
• W2046643103 cites W2058348572 @default.
• W2046643103 cites W2063943960 @default.
• W2046643103 cites W2085086076 @default.
• W2046643103 cites W2090402915 @default.
• W2046643103 cites W2092992129 @default.
• W2046643103 cites W2102082802 @default.
• W2046643103 cites W2124102028 @default.
• W2046643103 cites W2130378095 @default.
• W2046643103 cites W2132995356 @default.
• W2046643103 cites W2161884465 @default.
• W2046643103 cites W2346940686 @default.
• W2046643103 cites W4293247451 @default.
• W2046643103 doi "https://doi.org/10.1016/j.molbiopara.2006.06.007" @default.
• W2046643103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16872692" @default.
• W2046643103 hasPublicationYear "2006" @default.
• W2046643103 type Work @default.
• W2046643103 sameAs 2046643103 @default.
• W2046643103 citedByCount "22" @default.
• W2046643103 countsByYear W20466431032012 @default.
• W2046643103 countsByYear W20466431032013 @default.
• W2046643103 countsByYear W20466431032014 @default.
• W2046643103 countsByYear W20466431032016 @default.
• W2046643103 countsByYear W20466431032017 @default.
• W2046643103 countsByYear W20466431032021 @default.
• W2046643103 countsByYear W20466431032022 @default.
• W2046643103 countsByYear W20466431032023 @default.
• W2046643103 crossrefType "journal-article" @default.
• W2046643103 hasAuthorship W2046643103A5028707814 @default.
• W2046643103 hasAuthorship W2046643103A5056170287 @default.
• W2046643103 hasAuthorship W2046643103A5085984380 @default.
• W2046643103 hasConcept C104317684 @default.
• W2046643103 hasConcept C105702510 @default.
• W2046643103 hasConcept C145822097 @default.
• W2046643103 hasConcept C153911025 @default.
• W2046643103 hasConcept C18903297 @default.
• W2046643103 hasConcept C2777499811 @default.
• W2046643103 hasConcept C2778830712 @default.
• W2046643103 hasConcept C2778944004 @default.
• W2046643103 hasConcept C2779825395 @default.
• W2046643103 hasConcept C2780535491 @default.
• W2046643103 hasConcept C2911037157 @default.
• W2046643103 hasConcept C54355233 @default.
• W2046643103 hasConcept C55493867 @default.
• W2046643103 hasConcept C86803240 @default.
• W2046643103 hasConcept C90856448 @default.
• W2046643103 hasConcept C98274493 @default.
• W2046643103 hasConceptScore W2046643103C104317684 @default.
• W2046643103 hasConceptScore W2046643103C105702510 @default.
• W2046643103 hasConceptScore W2046643103C145822097 @default.
• W2046643103 hasConceptScore W2046643103C153911025 @default.
• W2046643103 hasConceptScore W2046643103C18903297 @default.
• W2046643103 hasConceptScore W2046643103C2777499811 @default.
• W2046643103 hasConceptScore W2046643103C2778830712 @default.
• W2046643103 hasConceptScore W2046643103C2778944004 @default.
• W2046643103 hasConceptScore W2046643103C2779825395 @default.
• W2046643103 hasConceptScore W2046643103C2780535491 @default.
• W2046643103 hasConceptScore W2046643103C2911037157 @default.
• W2046643103 hasConceptScore W2046643103C54355233 @default.
• W2046643103 hasConceptScore W2046643103C55493867 @default.
• W2046643103 hasConceptScore W2046643103C86803240 @default.
• W2046643103 hasConceptScore W2046643103C90856448 @default.
• W2046643103 hasConceptScore W2046643103C98274493 @default.
• W2046643103 hasIssue "1" @default.
• W2046643103 hasLocation W20466431031 @default.
• W2046643103 hasLocation W20466431032 @default.
• W2046643103 hasOpenAccess W2046643103 @default.
• W2046643103 hasPrimaryLocation W20466431031 @default.
• W2046643103 hasRelatedWork W1971446667 @default.
• W2046643103 hasRelatedWork W2029511489 @default.
• W2046643103 hasRelatedWork W2038638853 @default.

• next