FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046706040> ?p ?o ?g. }

• W2046706040 abstract "A challenge to antibody engineering is the large number of positions and nature of variation and opposing concerns of introducing unfavorable biochemical properties. While large libraries are quite successful in identifying antibodies with improved binding or activity, still only a fraction of possibilities can be explored and that would require considerable effort. The vast array of natural antibody sequences provides a potential wealth of information on (1) selecting hotspots for variation, and (2) designing mutants to mimic natural variations seen in hotspots. The human immune system can generate an enormous diversity of immunoglobulins against an almost unlimited range of antigens by gene rearrangement of a limited number of germline variable, diversity and joining genes followed by somatic hypermutation and antigen selection. All the antibody sequences in NCBI database can be assigned to different germline genes. As a result, a position specific scoring matrix for each germline gene can be constructed by aligning all its member sequences and calculating the amino acid frequencies for each position. The position specific scoring matrix for each germline gene characterizes hotspots and the nature of variations, and thus reduces the sequence space of exploration in antibody engineering. We have developed a bioinformatics pipeline to conduct analysis of human antibody sequences, and generated a comprehensive knowledge database for in silico antibody engineering. The pipeline is fully automatic and the knowledge database can be refreshed anytime by re-running the pipeline. The refresh process is fast, typically taking 1min on a Lenovo ThinkPad T60 laptop with 3G memory. Our knowledge database consists of (1) the individual germline gene usage in generation of natural antibodies; (2) the CDR length distributions; and (3) the position specific scoring matrix for each germline gene. The knowledge database provides comprehensive support for antibody engineering, including de novo library design in selection of favorable germline V gene scaffolds and CDR lengths. In addition, we have also developed a web application framework to present our knowledge database, and the web interface can help people to easily retrieve a variety of information from the knowledge database." @default.
• W2046706040 created "2016-06-24" @default.
• W2046706040 creator A5014628406 @default.
• W2046706040 creator A5044142602 @default.
• W2046706040 date "2011-04-01" @default.
• W2046706040 modified "2023-09-25" @default.
• W2046706040 title "A bioinformatics pipeline to build a knowledge database for in silico antibody engineering" @default.
• W2046706040 cites W1484092314 @default.
• W2046706040 cites W1972564604 @default.
• W2046706040 cites W1982679430 @default.
• W2046706040 cites W2000861313 @default.
• W2046706040 cites W2003131026 @default.
• W2046706040 cites W2004178565 @default.
• W2046706040 cites W2035802316 @default.
• W2046706040 cites W2047553972 @default.
• W2046706040 cites W2067001279 @default.
• W2046706040 cites W2078070662 @default.
• W2046706040 cites W2082947088 @default.
• W2046706040 cites W2094011807 @default.
• W2046706040 cites W2103735815 @default.
• W2046706040 cites W2105273832 @default.
• W2046706040 cites W2115122333 @default.
• W2046706040 cites W2120999180 @default.
• W2046706040 cites W2136892614 @default.
• W2046706040 cites W2159866133 @default.
• W2046706040 cites W2325792050 @default.
• W2046706040 cites W623638409 @default.
• W2046706040 doi "https://doi.org/10.1016/j.molimm.2011.01.009" @default.
• W2046706040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21310488" @default.
• W2046706040 hasPublicationYear "2011" @default.
• W2046706040 type Work @default.
• W2046706040 sameAs 2046706040 @default.
• W2046706040 citedByCount "1" @default.
• W2046706040 crossrefType "journal-article" @default.
• W2046706040 hasAuthorship W2046706040A5014628406 @default.
• W2046706040 hasAuthorship W2046706040A5044142602 @default.
• W2046706040 hasConcept C104317684 @default.
• W2046706040 hasConcept C109825262 @default.
• W2046706040 hasConcept C199360897 @default.
• W2046706040 hasConcept C2775905019 @default.
• W2046706040 hasConcept C41008148 @default.
• W2046706040 hasConcept C43521106 @default.
• W2046706040 hasConcept C54355233 @default.
• W2046706040 hasConcept C70721500 @default.
• W2046706040 hasConcept C77088390 @default.
• W2046706040 hasConcept C86803240 @default.
• W2046706040 hasConceptScore W2046706040C104317684 @default.
• W2046706040 hasConceptScore W2046706040C109825262 @default.
• W2046706040 hasConceptScore W2046706040C199360897 @default.
• W2046706040 hasConceptScore W2046706040C2775905019 @default.
• W2046706040 hasConceptScore W2046706040C41008148 @default.
• W2046706040 hasConceptScore W2046706040C43521106 @default.
• W2046706040 hasConceptScore W2046706040C54355233 @default.
• W2046706040 hasConceptScore W2046706040C70721500 @default.
• W2046706040 hasConceptScore W2046706040C77088390 @default.
• W2046706040 hasConceptScore W2046706040C86803240 @default.
• W2046706040 hasLocation W20467060401 @default.
• W2046706040 hasLocation W20467060402 @default.
• W2046706040 hasOpenAccess W2046706040 @default.
• W2046706040 hasPrimaryLocation W20467060401 @default.
• W2046706040 hasRelatedWork W1542541022 @default.
• W2046706040 hasRelatedWork W1802312377 @default.
• W2046706040 hasRelatedWork W1805167282 @default.
• W2046706040 hasRelatedWork W1890359263 @default.
• W2046706040 hasRelatedWork W1977828801 @default.
• W2046706040 hasRelatedWork W2004072996 @default.
• W2046706040 hasRelatedWork W2035634951 @default.
• W2046706040 hasRelatedWork W2067001279 @default.
• W2046706040 hasRelatedWork W2070906314 @default.
• W2046706040 hasRelatedWork W2077519340 @default.
• W2046706040 hasRelatedWork W2084123169 @default.
• W2046706040 hasRelatedWork W2090676009 @default.
• W2046706040 hasRelatedWork W2140960177 @default.
• W2046706040 hasRelatedWork W2144725991 @default.
• W2046706040 hasRelatedWork W2153710191 @default.
• W2046706040 hasRelatedWork W2171320586 @default.
• W2046706040 hasRelatedWork W2326384723 @default.
• W2046706040 hasRelatedWork W263186169 @default.
• W2046706040 hasRelatedWork W3145013805 @default.
• W2046706040 hasRelatedWork W68739067 @default.
• W2046706040 isParatext "false" @default.
• W2046706040 isRetracted "false" @default.
• W2046706040 magId "2046706040" @default.
• W2046706040 workType "article" @default.