Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046792908> ?p ?o ?g. }
- W2046792908 endingPage "597" @default.
- W2046792908 startingPage "587" @default.
- W2046792908 abstract "Vitellogenin was purified from plasma of estradiol-17β-treated cod (Gadus morhua) rainbow trout (Oncorhynchus mykiss), turbot (Scophthalmus maximus), and wolffish (Anarhichas lupus) by precipitation with EDTA:Mg2+, distilled water, and high-performance ion-exchange chromatography. Vitellogenin of high purity was obtained by precipitation followed by chromatography, as evaluated by an homologous antiserum developed for each species. The instability of vitellogenin demanded consistent low temperature and the use of protease inhibitor before blood sampling. When the necessary precautions were taken, vitellogenin from rainbow trout, turbot, and wolffish eluted as one regular peak during chromatography. Cod vitellogenin eluted as two peaks and these demonstrated identical migration patterns on SDS-PAGE. The observed differences in stability between the four species suggest that isolation procedures should be modified according to the requirements for each species. Electrophoresis of plasma from treated fish revealed the presence of several smaller proteins, with a molecular mass around 50 kDa, that were considered to be vitelline envelope proteins. Other minor plasma proteins were immunoreactive to antisera, directed against vitellogenin and therefore judged to be fragments of degraded vitellogenin. © 1993 Wiley-Liss, Inc." @default.
- W2046792908 created "2016-06-24" @default.
- W2046792908 creator A5040485799 @default.
- W2046792908 creator A5057837076 @default.
- W2046792908 creator A5068900582 @default.
- W2046792908 date "1993-12-15" @default.
- W2046792908 modified "2023-10-09" @default.
- W2046792908 title "Isolation, immunochemical detection, and observations of the instability of vitellogenin from four teleosts" @default.
- W2046792908 cites W1489202660 @default.
- W2046792908 cites W1513688351 @default.
- W2046792908 cites W1539299047 @default.
- W2046792908 cites W1552816972 @default.
- W2046792908 cites W1964083742 @default.
- W2046792908 cites W1964211295 @default.
- W2046792908 cites W1964848007 @default.
- W2046792908 cites W1967350623 @default.
- W2046792908 cites W1968833692 @default.
- W2046792908 cites W1970583103 @default.
- W2046792908 cites W1976139878 @default.
- W2046792908 cites W1980048959 @default.
- W2046792908 cites W1991883403 @default.
- W2046792908 cites W2004731710 @default.
- W2046792908 cites W2005742504 @default.
- W2046792908 cites W2014238640 @default.
- W2046792908 cites W2020858580 @default.
- W2046792908 cites W2040530362 @default.
- W2046792908 cites W2051701399 @default.
- W2046792908 cites W2063089960 @default.
- W2046792908 cites W2067183684 @default.
- W2046792908 cites W2075903299 @default.
- W2046792908 cites W2088347279 @default.
- W2046792908 cites W2092920707 @default.
- W2046792908 cites W2100837269 @default.
- W2046792908 cites W2101108802 @default.
- W2046792908 cites W2140213056 @default.
- W2046792908 cites W2314317407 @default.
- W2046792908 cites W2330120646 @default.
- W2046792908 cites W4252960083 @default.
- W2046792908 doi "https://doi.org/10.1002/jez.1402670606" @default.
- W2046792908 hasPublicationYear "1993" @default.
- W2046792908 type Work @default.
- W2046792908 sameAs 2046792908 @default.
- W2046792908 citedByCount "98" @default.
- W2046792908 countsByYear W20467929082012 @default.
- W2046792908 countsByYear W20467929082013 @default.
- W2046792908 countsByYear W20467929082014 @default.
- W2046792908 countsByYear W20467929082016 @default.
- W2046792908 countsByYear W20467929082017 @default.
- W2046792908 countsByYear W20467929082018 @default.
- W2046792908 countsByYear W20467929082019 @default.
- W2046792908 countsByYear W20467929082021 @default.
- W2046792908 countsByYear W20467929082022 @default.
- W2046792908 crossrefType "journal-article" @default.
- W2046792908 hasAuthorship W2046792908A5040485799 @default.
- W2046792908 hasAuthorship W2046792908A5057837076 @default.
- W2046792908 hasAuthorship W2046792908A5068900582 @default.
- W2046792908 hasConcept C104317684 @default.
- W2046792908 hasConcept C153911025 @default.
- W2046792908 hasConcept C179247698 @default.
- W2046792908 hasConcept C181199279 @default.
- W2046792908 hasConcept C185592680 @default.
- W2046792908 hasConcept C2777883778 @default.
- W2046792908 hasConcept C2779078696 @default.
- W2046792908 hasConcept C2779282177 @default.
- W2046792908 hasConcept C2909208804 @default.
- W2046792908 hasConcept C2994167347 @default.
- W2046792908 hasConcept C43617362 @default.
- W2046792908 hasConcept C505870484 @default.
- W2046792908 hasConcept C55493867 @default.
- W2046792908 hasConcept C86803240 @default.
- W2046792908 hasConceptScore W2046792908C104317684 @default.
- W2046792908 hasConceptScore W2046792908C153911025 @default.
- W2046792908 hasConceptScore W2046792908C179247698 @default.
- W2046792908 hasConceptScore W2046792908C181199279 @default.
- W2046792908 hasConceptScore W2046792908C185592680 @default.
- W2046792908 hasConceptScore W2046792908C2777883778 @default.
- W2046792908 hasConceptScore W2046792908C2779078696 @default.
- W2046792908 hasConceptScore W2046792908C2779282177 @default.
- W2046792908 hasConceptScore W2046792908C2909208804 @default.
- W2046792908 hasConceptScore W2046792908C2994167347 @default.
- W2046792908 hasConceptScore W2046792908C43617362 @default.
- W2046792908 hasConceptScore W2046792908C505870484 @default.
- W2046792908 hasConceptScore W2046792908C55493867 @default.
- W2046792908 hasConceptScore W2046792908C86803240 @default.
- W2046792908 hasIssue "6" @default.
- W2046792908 hasLocation W20467929081 @default.
- W2046792908 hasOpenAccess W2046792908 @default.
- W2046792908 hasPrimaryLocation W20467929081 @default.
- W2046792908 hasRelatedWork W1978724027 @default.
- W2046792908 hasRelatedWork W1984944208 @default.
- W2046792908 hasRelatedWork W2000978012 @default.
- W2046792908 hasRelatedWork W2004392914 @default.
- W2046792908 hasRelatedWork W2006601145 @default.
- W2046792908 hasRelatedWork W2012465845 @default.
- W2046792908 hasRelatedWork W2167725900 @default.
- W2046792908 hasRelatedWork W2384523798 @default.
- W2046792908 hasRelatedWork W2393822016 @default.
- W2046792908 hasRelatedWork W4293268785 @default.