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• W2046803513 abstract "Nutrition modulates both production and composition of milk. Milk composition was studied in rats chronically fed a diet without additional lipids, and therefore eating only traces of the recommended supply of essential polyunsaturated fatty acid. Despite a large decrease in milk-protein synthesis, only protein composition, but not protein concentration, was found to change in the milk of rats following a lipid-deprived diet. Correlatively, we observed a substantial increase in the lactose concentration of milk. Analysis of milk proteins by two-dimensional electrophoresis demonstrated that the relative proportion of the various molecular forms of κ-casein, an O-glycosylated protein, was modified in the milk of rats receiving the lipid-deprived diet. In tissues, differences in the two-dimensional pattern of κ-casein between control and lipid-deprived rats were similar, if not identical. In contrast to κ-casein, the molecular forms of α-lactalbumin, an N-glycosylated protein, were not affected by the diet. These data provide evidence that O-glycosylation of milk proteins in the secretory pathway of mammary epithelial cells is modulated by the lipid content of experimental diets." @default.
• W2046803513 created "2016-06-24" @default.
• W2046803513 creator A5009649825 @default.
• W2046803513 creator A5077193639 @default.
• W2046803513 date "2008-01-01" @default.
• W2046803513 modified "2023-10-17" @default.
• W2046803513 title "Lipid-deprived diet perturbs O-glycosylation of secretory proteins in rat mammary epithelial cells" @default.
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• W2046803513 doi "https://doi.org/10.1017/s1751731107001309" @default.
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• W2046803513 hasPublicationYear "2008" @default.
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