Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046896503> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2046896503 endingPage "M444" @default.
- W2046896503 startingPage "M441" @default.
- W2046896503 abstract "To improve the culture conditions of hepatocytes for use as a bioartificial liver, the effects of shear flow on the co-culture system of hepatocytes/nonparenchymal cells (NPC) were investigated. A flow chamber with a collagen coated rectangular glass plate, where hepatocytes (5 × 104 cell/cm2) and NPC (2 × 105 cell/cm2) were seeded, was used to attain a shear stress of 4.7 dyne/cm2. Concentrations of ammonia and urea in the medium were measured daily during the 2 week experiment. The metabolic activity of hepatocytes in the homotypic culture were lower than those of the co-culture, especially when the cultivation time exceeded 1 week. In addition, the applied shear flow promoted activity of the co-culture system. An enhancement in the rates of ammonium removal and urea synthesis was obtained in the perfusion systems. Morphologic observation revealed that aggregates of hepatocytes formed abundantly in the perfusion system and hepatocytes developed a cuboid shape. This suggested that perfusion affected the function and morphology of hepatocytes in the co-culture system. Shear flow could induce cell-cell interactions and secretion of extracellular matrix through the activation of NPC." @default.
- W2046896503 created "2016-06-24" @default.
- W2046896503 creator A5006284018 @default.
- W2046896503 creator A5031475164 @default.
- W2046896503 creator A5032459512 @default.
- W2046896503 creator A5079708681 @default.
- W2046896503 date "1998-09-01" @default.
- W2046896503 modified "2023-10-17" @default.
- W2046896503 title "Effects of Shear Stress on Metabolic Function of the Co-Culture System of Hepatocyte/Nonparenchymal Cells for a Bioartificial Liver" @default.
- W2046896503 doi "https://doi.org/10.1097/00002480-199809000-00023" @default.
- W2046896503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9804468" @default.
- W2046896503 hasPublicationYear "1998" @default.
- W2046896503 type Work @default.
- W2046896503 sameAs 2046896503 @default.
- W2046896503 citedByCount "49" @default.
- W2046896503 countsByYear W20468965032013 @default.
- W2046896503 countsByYear W20468965032014 @default.
- W2046896503 countsByYear W20468965032015 @default.
- W2046896503 countsByYear W20468965032016 @default.
- W2046896503 countsByYear W20468965032017 @default.
- W2046896503 countsByYear W20468965032018 @default.
- W2046896503 countsByYear W20468965032019 @default.
- W2046896503 countsByYear W20468965032020 @default.
- W2046896503 countsByYear W20468965032021 @default.
- W2046896503 crossrefType "journal-article" @default.
- W2046896503 hasAuthorship W2046896503A5006284018 @default.
- W2046896503 hasAuthorship W2046896503A5031475164 @default.
- W2046896503 hasAuthorship W2046896503A5032459512 @default.
- W2046896503 hasAuthorship W2046896503A5079708681 @default.
- W2046896503 hasConcept C12554922 @default.
- W2046896503 hasConcept C126322002 @default.
- W2046896503 hasConcept C136229726 @default.
- W2046896503 hasConcept C146957229 @default.
- W2046896503 hasConcept C159985019 @default.
- W2046896503 hasConcept C185592680 @default.
- W2046896503 hasConcept C189165786 @default.
- W2046896503 hasConcept C192562407 @default.
- W2046896503 hasConcept C202751555 @default.
- W2046896503 hasConcept C21141959 @default.
- W2046896503 hasConcept C2776200302 @default.
- W2046896503 hasConcept C2780365088 @default.
- W2046896503 hasConcept C28406088 @default.
- W2046896503 hasConcept C54355233 @default.
- W2046896503 hasConcept C55493867 @default.
- W2046896503 hasConcept C71867830 @default.
- W2046896503 hasConcept C71924100 @default.
- W2046896503 hasConcept C81885089 @default.
- W2046896503 hasConcept C86803240 @default.
- W2046896503 hasConcept C95444343 @default.
- W2046896503 hasConceptScore W2046896503C12554922 @default.
- W2046896503 hasConceptScore W2046896503C126322002 @default.
- W2046896503 hasConceptScore W2046896503C136229726 @default.
- W2046896503 hasConceptScore W2046896503C146957229 @default.
- W2046896503 hasConceptScore W2046896503C159985019 @default.
- W2046896503 hasConceptScore W2046896503C185592680 @default.
- W2046896503 hasConceptScore W2046896503C189165786 @default.
- W2046896503 hasConceptScore W2046896503C192562407 @default.
- W2046896503 hasConceptScore W2046896503C202751555 @default.
- W2046896503 hasConceptScore W2046896503C21141959 @default.
- W2046896503 hasConceptScore W2046896503C2776200302 @default.
- W2046896503 hasConceptScore W2046896503C2780365088 @default.
- W2046896503 hasConceptScore W2046896503C28406088 @default.
- W2046896503 hasConceptScore W2046896503C54355233 @default.
- W2046896503 hasConceptScore W2046896503C55493867 @default.
- W2046896503 hasConceptScore W2046896503C71867830 @default.
- W2046896503 hasConceptScore W2046896503C71924100 @default.
- W2046896503 hasConceptScore W2046896503C81885089 @default.
- W2046896503 hasConceptScore W2046896503C86803240 @default.
- W2046896503 hasConceptScore W2046896503C95444343 @default.
- W2046896503 hasIssue "5" @default.
- W2046896503 hasLocation W20468965031 @default.
- W2046896503 hasLocation W20468965032 @default.
- W2046896503 hasOpenAccess W2046896503 @default.
- W2046896503 hasPrimaryLocation W20468965031 @default.
- W2046896503 hasRelatedWork W1801283623 @default.
- W2046896503 hasRelatedWork W2033548804 @default.
- W2046896503 hasRelatedWork W2147002266 @default.
- W2046896503 hasRelatedWork W2255019515 @default.
- W2046896503 hasRelatedWork W2351635801 @default.
- W2046896503 hasRelatedWork W2389453651 @default.
- W2046896503 hasRelatedWork W2397931218 @default.
- W2046896503 hasRelatedWork W2411030177 @default.
- W2046896503 hasRelatedWork W2493017073 @default.
- W2046896503 hasRelatedWork W2793858243 @default.
- W2046896503 hasVolume "44" @default.
- W2046896503 isParatext "false" @default.
- W2046896503 isRetracted "false" @default.
- W2046896503 magId "2046896503" @default.
- W2046896503 workType "article" @default.