FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046952308> ?p ?o ?g. }

• W2046952308 endingPage "2775" @default.
• W2046952308 startingPage "2775" @default.
• W2046952308 abstract "The authors have recently demonstrated that substance P and L-733,060 induce cell proliferation and cell inhibition, respectively, in human retinoblastoma cell lines. However, the presence of neurokinin-1 receptors has not been demonstrated in such cell lines, nor is it known whether other neurokinin-1 receptor antagonists exert antitumoral action against retinoblastoma cell lines. The purpose of this study was to demonstrate the presence of neurokinin-1 receptors in the human retinoblastoma cell lines WERI-Rb-1 and Y-79 and to study the growth inhibitory capacity of the neurokinin-1 receptor antagonist L-732,138 against those cell lines. The authors also sought to demonstrate that the administration of L-732,138 or L-733,060 induces apoptosis in retinoblastoma cells and that neurokinin-1 receptors and substance P are present in primary retinoblastoma.Immunoblot analysis was used to determine neurokinin-1 receptors, and a Coulter counter was used to determine viable cell numbers; this was followed by application of the tetrazolium compound WST-8, a colorimetric method, to evaluate cell viability. DAPI stain was applied to assess chromatin condensation, characteristic of apoptosis, and immunoperoxidase was used to demonstrate neurokinin-1 receptors and substance P in eyes with primary retinoblastoma.Neurokinin-1 receptors were present in both retinoblastoma cell lines studied. Three identical bands (isoforms of approximately 33, 58, and 75 kDa) were observed in both cell lines. Moreover, L-732,138 inhibited the growth of both cell lines studied, with and without previous administration of substance P. This inhibition occurred in a dose-dependent manner, with the IC50 values of 60.47 microM for WERI-Rb1 and 56.78 microM for Y-79. Moreover, apoptosis was observed in both cell lines after the administration of L-732,138 or L-733,060. In fixed eyes with primary retinoblastoma, a high density of neurokinin-1 receptors was observed in tumor cells, whereas a very low number of such cells contained substance P.This study showed that the same isoforms of the neurokinin-1 receptor are present in human retinoblastoma cell lines WERI-Rb-1 and Y-79. Both L-732,138 and L-733,060 can induce apoptosis in these cell lines and therefore can act as antitumoral agents. Primary retinoblastoma specimens display neurokinin-1 receptor immunolabeling. These results suggest that the neurokinin-1 receptor may be a promising new target for the treatment of retinoblastoma." @default.
• W2046952308 created "2016-06-24" @default.
• W2046952308 creator A5020163776 @default.
• W2046952308 creator A5049301955 @default.
• W2046952308 creator A5050837370 @default.
• W2046952308 creator A5057284941 @default.
• W2046952308 creator A5068838039 @default.
• W2046952308 creator A5090666491 @default.
• W2046952308 date "2007-06-01" @default.
• W2046952308 modified "2023-10-14" @default.
• W2046952308 title "Neurokinin-1 Receptors Located in Human Retinoblastoma Cell Lines: Antitumor Action of Its Antagonist, L-732,138" @default.
• W2046952308 cites W134871497 @default.
• W2046952308 cites W1488537054 @default.
• W2046952308 cites W1489345976 @default.
• W2046952308 cites W1567537312 @default.
• W2046952308 cites W1632492912 @default.
• W2046952308 cites W1965108104 @default.
• W2046952308 cites W1965186631 @default.
• W2046952308 cites W1972006208 @default.
• W2046952308 cites W1973773456 @default.
• W2046952308 cites W1979982794 @default.
• W2046952308 cites W1983456015 @default.
• W2046952308 cites W1995725538 @default.
• W2046952308 cites W2009158977 @default.
• W2046952308 cites W201820481 @default.
• W2046952308 cites W2018968319 @default.
• W2046952308 cites W2020835847 @default.
• W2046952308 cites W2026255554 @default.
• W2046952308 cites W2040354737 @default.
• W2046952308 cites W2049743016 @default.
• W2046952308 cites W2050399061 @default.
• W2046952308 cites W2054222308 @default.
• W2046952308 cites W2058523849 @default.
• W2046952308 cites W2059588630 @default.
• W2046952308 cites W2059780291 @default.
• W2046952308 cites W2069306418 @default.
• W2046952308 cites W2089181120 @default.
• W2046952308 cites W2105620605 @default.
• W2046952308 cites W2114308408 @default.
• W2046952308 cites W2130268591 @default.
• W2046952308 cites W2137141980 @default.
• W2046952308 cites W2145234243 @default.
• W2046952308 cites W2152552931 @default.
• W2046952308 cites W76344694 @default.
• W2046952308 cites W2094525939 @default.
• W2046952308 doi "https://doi.org/10.1167/iovs.05-1591" @default.
• W2046952308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17525212" @default.
• W2046952308 hasPublicationYear "2007" @default.
• W2046952308 type Work @default.
• W2046952308 sameAs 2046952308 @default.
• W2046952308 citedByCount "55" @default.
• W2046952308 countsByYear W20469523082012 @default.
• W2046952308 countsByYear W20469523082013 @default.
• W2046952308 countsByYear W20469523082014 @default.
• W2046952308 countsByYear W20469523082015 @default.
• W2046952308 countsByYear W20469523082016 @default.
• W2046952308 countsByYear W20469523082017 @default.
• W2046952308 countsByYear W20469523082018 @default.
• W2046952308 countsByYear W20469523082019 @default.
• W2046952308 countsByYear W20469523082020 @default.
• W2046952308 countsByYear W20469523082021 @default.
• W2046952308 countsByYear W20469523082022 @default.
• W2046952308 countsByYear W20469523082023 @default.
• W2046952308 crossrefType "journal-article" @default.
• W2046952308 hasAuthorship W2046952308A5020163776 @default.
• W2046952308 hasAuthorship W2046952308A5049301955 @default.
• W2046952308 hasAuthorship W2046952308A5050837370 @default.
• W2046952308 hasAuthorship W2046952308A5057284941 @default.
• W2046952308 hasAuthorship W2046952308A5068838039 @default.
• W2046952308 hasAuthorship W2046952308A5090666491 @default.
• W2046952308 hasBestOaLocation W20469523081 @default.
• W2046952308 hasConcept C104317684 @default.
• W2046952308 hasConcept C118303440 @default.
• W2046952308 hasConcept C137130169 @default.
• W2046952308 hasConcept C1491633281 @default.
• W2046952308 hasConcept C153911025 @default.
• W2046952308 hasConcept C170493617 @default.
• W2046952308 hasConcept C185592680 @default.
• W2046952308 hasConcept C2776533618 @default.
• W2046952308 hasConcept C2776577112 @default.
• W2046952308 hasConcept C2777056410 @default.
• W2046952308 hasConcept C54355233 @default.
• W2046952308 hasConcept C55493867 @default.
• W2046952308 hasConcept C62112901 @default.
• W2046952308 hasConcept C81885089 @default.
• W2046952308 hasConcept C86803240 @default.
• W2046952308 hasConcept C95444343 @default.
• W2046952308 hasConceptScore W2046952308C104317684 @default.
• W2046952308 hasConceptScore W2046952308C118303440 @default.
• W2046952308 hasConceptScore W2046952308C137130169 @default.
• W2046952308 hasConceptScore W2046952308C1491633281 @default.
• W2046952308 hasConceptScore W2046952308C153911025 @default.
• W2046952308 hasConceptScore W2046952308C170493617 @default.
• W2046952308 hasConceptScore W2046952308C185592680 @default.
• W2046952308 hasConceptScore W2046952308C2776533618 @default.
• W2046952308 hasConceptScore W2046952308C2776577112 @default.
• W2046952308 hasConceptScore W2046952308C2777056410 @default.
• W2046952308 hasConceptScore W2046952308C54355233 @default.

• next