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- W2047044089 abstract "No transport against a concentration gradient occurred using the hamster everted small intestine in vitro if the COOH group of an α-amino acid was replaced by CH2OH, CH3, CO·C6H5, or CONHOH. Transport apparently occurred when the replacing group was CO·NH2. Evidence is presented that in these cases deamination or transamination (producing the free amino acid) probably preceded transinestinal transport of the compound. [2-14C]Glycine methyl ester hydrochloride, purified to free it of glycine, was not transported by everted intestinal sacs from hamster, rat or chinchilla. There was thus no appreciable esterase activity under these conditions. Three phosphonic acid analogues (RCHNH2·PO3H2) did not inhibit amino acid movement (suggesting PO3H2 cannot replace COOH). A sulfonic acid analogue (RCHNH2·SO3H) was not completely stable under these conditions; it was not transported, and was not a transport inhibitor. Aminoiminomethane sulfinic acid also did not inhibit transport. Carboxylic acid alone, and amines plus carboxylic acids, were not effective inhibitors of amino acid transport. An −SO3− or a second COO− group on the amino acid converted a transported molecule into one that was not transported and also did not inhibit amino acid movement." @default.
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- W2047044089 date "1966-04-01" @default.
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- W2047044089 title "Role of the carboxyl group in intestinal amino acid transport" @default.
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- W2047044089 doi "https://doi.org/10.1016/0304-4165(66)90092-4" @default.
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