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- W2047091978 abstract "Steroid transport in Pseudomonas testosteroni membrane vesicles was significantly inhibited by the uncoupled 2,4-dinitrophenol (DNP). Inhibition of steroid transport was not due to inhibition of the 3 beta- and 17 beta-hydroxysteroid dehydrogenase by concentrations of up to 1 mM DNP. However, inhibition of this membrane-bound enzyme was measured at 10 mM DNP. The solubilized 3 beta- and 17 beta-hydroxysteroid dehydrogenase was more sensitive, being inhibited at both 1 and 10 mM DNP indicating a specific inhibition of this enzyme by DNP. Testosterone-dependent oxygen consumption was stimulated slightly at low concentrations of DNP and inhibited at high concentrations. The inhibition of testosterone-dependent oxygen consumption correlated with the inhibition of transport. This indicated that the inhibition of transport by DNP was due to a direct inhibition of metabolism. The existence of an electrochemical gradient is used to explain these results." @default.
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- W2047091978 date "1983-03-01" @default.
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- W2047091978 title "The effect of 2,4-Dinitrophenol on steroid transport in membrane vesicles of Pseudomonas testosteroni" @default.
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- W2047091978 doi "https://doi.org/10.1016/0022-4731(83)90118-8" @default.
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