FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047138766> ?p ?o ?g. }

• W2047138766 endingPage "118" @default.
• W2047138766 startingPage "105" @default.
• W2047138766 abstract "The purpose of this investigation is to characterize the reduced state of RNase A (r-RNase A) in terms of (i) intramolecular distances, (ii) the sequence of formation of stable loops in the initial stages of folding, and (iii) the unfolding transitions induced by GdnHCl. This is accomplished by identifying specific subdomain structures and local and long-range interactions that direct the folding process of this protein and lead to the native fold and formation of the disulfide bonds. Eleven pairs of dispersed sites in the RNase A molecule were labeled with fluorescent donor and acceptor probes, and the distributions of intramolecular distances (IDDs) were determined by means of time-resolved dynamic nonradiative excitation energy transfer (TR-FRET) measurements. The mutants were designed to search for (a) a possible nonrandom fold of the backbone in the collapsed state and (b) possible loops stabilized by long-range interactions. It was found that, under folding conditions, (i) the labeled mutants of r-RNase A in refolding buffer (the R(N) state) exhibit features of specific (nonrandom) compact but very dispersed subdomain structures (indicated by short mean distances, broad IDDs, and a weak dependence of the mean distances on segment length), (ii) the backbone fold in the C-terminal beta-like portion of the molecule appears to adopt a native-like overall fold, (iii) the N-terminal alpha-like portion of the chain is separated from the C-terminal core by very large intramolecular distances, larger than those in the crystal structure, and (iv) perturbations by addition of GdnHCl reveal several conformational transitions in different sections of the chain. Addition of GdnHCl to the native disulfide-intact protein provided a reference state for the extent of expansion of intramolecular distances under denaturing conditions. In conclusion, r-RNase A under folding conditions (the R(N) state) is poised for the final folding step(s) with a native-like trace of the chain fold but a large separation between the two subdomains which is then decreased upon introduction of three of the four native disulfide cross-links." @default.
• W2047138766 created "2016-06-24" @default.
• W2047138766 creator A5022665888 @default.
• W2047138766 creator A5022677369 @default.
• W2047138766 creator A5058608549 @default.
• W2047138766 creator A5072853154 @default.
• W2047138766 creator A5082572644 @default.
• W2047138766 date "2000-12-13" @default.
• W2047138766 modified "2023-09-23" @default.
• W2047138766 title "Distributions of Intramolecular Distances in the Reduced and Denatured States of Bovine Pancreatic Ribonuclease A. Folding Initiation Structures in the C-Terminal Portions of the Reduced Protein" @default.
• W2047138766 cites W1969163423 @default.
• W2047138766 cites W1969178181 @default.
• W2047138766 cites W1999479012 @default.
• W2047138766 cites W2025264553 @default.
• W2047138766 cites W2042243687 @default.
• W2047138766 cites W2046969603 @default.
• W2047138766 cites W2078870197 @default.
• W2047138766 cites W2123072799 @default.
• W2047138766 doi "https://doi.org/10.1021/bi001946o" @default.
• W2047138766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11141061" @default.
• W2047138766 hasPublicationYear "2000" @default.
• W2047138766 type Work @default.
• W2047138766 sameAs 2047138766 @default.
• W2047138766 citedByCount "77" @default.
• W2047138766 countsByYear W20471387662012 @default.
• W2047138766 countsByYear W20471387662013 @default.
• W2047138766 countsByYear W20471387662014 @default.
• W2047138766 countsByYear W20471387662016 @default.
• W2047138766 countsByYear W20471387662017 @default.
• W2047138766 countsByYear W20471387662018 @default.
• W2047138766 countsByYear W20471387662020 @default.
• W2047138766 countsByYear W20471387662021 @default.
• W2047138766 countsByYear W20471387662022 @default.
• W2047138766 crossrefType "journal-article" @default.
• W2047138766 hasAuthorship W2047138766A5022665888 @default.
• W2047138766 hasAuthorship W2047138766A5022677369 @default.
• W2047138766 hasAuthorship W2047138766A5058608549 @default.
• W2047138766 hasAuthorship W2047138766A5072853154 @default.
• W2047138766 hasAuthorship W2047138766A5082572644 @default.
• W2047138766 hasConcept C104317684 @default.
• W2047138766 hasConcept C10879258 @default.
• W2047138766 hasConcept C119599485 @default.
• W2047138766 hasConcept C121332964 @default.
• W2047138766 hasConcept C127413603 @default.
• W2047138766 hasConcept C178790620 @default.
• W2047138766 hasConcept C185592680 @default.
• W2047138766 hasConcept C19472624 @default.
• W2047138766 hasConcept C204328495 @default.
• W2047138766 hasConcept C2776545253 @default.
• W2047138766 hasConcept C2776813977 @default.
• W2047138766 hasConcept C2778976237 @default.
• W2047138766 hasConcept C32909587 @default.
• W2047138766 hasConcept C55493867 @default.
• W2047138766 hasConcept C62520636 @default.
• W2047138766 hasConcept C67705224 @default.
• W2047138766 hasConcept C71240020 @default.
• W2047138766 hasConcept C75079739 @default.
• W2047138766 hasConcept C8010536 @default.
• W2047138766 hasConcept C91881484 @default.
• W2047138766 hasConcept C96305047 @default.
• W2047138766 hasConceptScore W2047138766C104317684 @default.
• W2047138766 hasConceptScore W2047138766C10879258 @default.
• W2047138766 hasConceptScore W2047138766C119599485 @default.
• W2047138766 hasConceptScore W2047138766C121332964 @default.
• W2047138766 hasConceptScore W2047138766C127413603 @default.
• W2047138766 hasConceptScore W2047138766C178790620 @default.
• W2047138766 hasConceptScore W2047138766C185592680 @default.
• W2047138766 hasConceptScore W2047138766C19472624 @default.
• W2047138766 hasConceptScore W2047138766C204328495 @default.
• W2047138766 hasConceptScore W2047138766C2776545253 @default.
• W2047138766 hasConceptScore W2047138766C2776813977 @default.
• W2047138766 hasConceptScore W2047138766C2778976237 @default.
• W2047138766 hasConceptScore W2047138766C32909587 @default.
• W2047138766 hasConceptScore W2047138766C55493867 @default.
• W2047138766 hasConceptScore W2047138766C62520636 @default.
• W2047138766 hasConceptScore W2047138766C67705224 @default.
• W2047138766 hasConceptScore W2047138766C71240020 @default.
• W2047138766 hasConceptScore W2047138766C75079739 @default.
• W2047138766 hasConceptScore W2047138766C8010536 @default.
• W2047138766 hasConceptScore W2047138766C91881484 @default.
• W2047138766 hasConceptScore W2047138766C96305047 @default.
• W2047138766 hasIssue "1" @default.
• W2047138766 hasLocation W20471387661 @default.
• W2047138766 hasLocation W20471387662 @default.
• W2047138766 hasOpenAccess W2047138766 @default.
• W2047138766 hasPrimaryLocation W20471387661 @default.
• W2047138766 hasRelatedWork W1966893941 @default.
• W2047138766 hasRelatedWork W1980045199 @default.
• W2047138766 hasRelatedWork W1987096232 @default.
• W2047138766 hasRelatedWork W1992173219 @default.
• W2047138766 hasRelatedWork W2002976844 @default.
• W2047138766 hasRelatedWork W2030866191 @default.
• W2047138766 hasRelatedWork W2060746978 @default.
• W2047138766 hasRelatedWork W2131604629 @default.
• W2047138766 hasRelatedWork W2133428998 @default.
• W2047138766 hasRelatedWork W3213711177 @default.
• W2047138766 hasVolume "40" @default.
• W2047138766 isParatext "false" @default.

• next