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- W2047143807 abstract "Phospholipase A 2 (PLA 2 ) is an enzyme that cleaves fatty acids from the sn -2 position of membrane phospholipids. Intracellular PLA 2 has been shown to be important for the generation of arachidonic acid, which leads to the synthesis of a wide range of eicosanoid paracrine hormones. Furthermore, secretory PLA 2 in the serum has been reported to exhibit important immune activities. In this study, BODIPY™ FL C 16 was used as a fluorescent probe to measure PLA 2 activity in the serum of the American alligator. The activity measured was dependent on the amounts of serum and labeled bacteria incubated. The phospholipase activity was also time-dependent, with near peak activities observed within 20–30 min. In addition, the observed activity was titrated away by increasing concentrations of unlabeled bacteria, indicating that the activity observed was specifically due to the presence of PLA 2 . The assay provides rapid results and requires only small amounts of serum." @default.
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- W2047143807 date "2009-01-01" @default.
- W2047143807 modified "2023-09-24" @default.
- W2047143807 title "A rapid fluorometric assay for the determination of serum phospholipase A2 activity" @default.
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- W2047143807 doi "https://doi.org/10.1016/j.microc.2008.08.006" @default.
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