FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047200410> ?p ?o ?g. }

• W2047200410 endingPage "401" @default.
• W2047200410 startingPage "385" @default.
• W2047200410 abstract "We present a study aimed at understanding the factors affecting the separation of large DNA molecules by capillary electrophoresis in polymer solutions. In a first series of experiments, a systematic study of the effect of operational parameters on the development of an electrohydrodynamic instability resulting in DNA aggregation and spurious peaks in the electropherograms is presented. The results are discussed in regard to a recent theory of electrohydrodynamic instabilities in macroion suspensions, recently proposed by Isambert et al. Overall, the results provide strong support to the theory. Some situations of interest for applications, and not explicitly considered in the theory, such as asymmetric field pulsing and the use of polymer additives in the buffer, were also considered. Furthermore, robust optimized protocols for high resolution separation of DNA in the range of 100 base pairs to 160 kilobase pairs, are proposed. As predicted by the model, it is shown that using a concentrated isoelectric buffer (histidine) strongly reduces aggregation as compared to the use of a conventional buffer at the same concentration, and allows separation of DNA from 100 bp to 160 kbp in less than 10 min. We also present a systematic study of the dependence of the mobility vs DNA size, pulse frequency, and field strength. The results are discussed with respect to the Biased Reptation with Fluctuations model and a good agreement is obtained. © 1999 John Wiley & Sons, Inc. Biopoly 49: 385–401, 1999" @default.
• W2047200410 created "2016-06-24" @default.
• W2047200410 creator A5032968167 @default.
• W2047200410 creator A5051021686 @default.
• W2047200410 creator A5067005790 @default.
• W2047200410 creator A5086939392 @default.
• W2047200410 date "1999-04-15" @default.
• W2047200410 modified "2023-09-24" @default.
• W2047200410 title "Electrohydrodynamically induced aggregation during constant and pulsed field capillary electrophoresis of DNA" @default.
• W2047200410 cites W1967302442 @default.
• W2047200410 cites W1983730464 @default.
• W2047200410 cites W1992197239 @default.
• W2047200410 cites W1992417490 @default.
• W2047200410 cites W2005697722 @default.
• W2047200410 cites W2009417020 @default.
• W2047200410 cites W2013404388 @default.
• W2047200410 cites W2015988576 @default.
• W2047200410 cites W2021329303 @default.
• W2047200410 cites W2022111622 @default.
• W2047200410 cites W2023183067 @default.
• W2047200410 cites W2029611498 @default.
• W2047200410 cites W2029681484 @default.
• W2047200410 cites W2030410834 @default.
• W2047200410 cites W2031682614 @default.
• W2047200410 cites W2034844564 @default.
• W2047200410 cites W2043128912 @default.
• W2047200410 cites W2043860143 @default.
• W2047200410 cites W2048039010 @default.
• W2047200410 cites W2053040199 @default.
• W2047200410 cites W2055912147 @default.
• W2047200410 cites W2058704759 @default.
• W2047200410 cites W2061140637 @default.
• W2047200410 cites W2061541601 @default.
• W2047200410 cites W2062255166 @default.
• W2047200410 cites W2085469845 @default.
• W2047200410 cites W2092093306 @default.
• W2047200410 cites W2094646376 @default.
• W2047200410 cites W2122799357 @default.
• W2047200410 cites W2149335063 @default.
• W2047200410 cites W2155997525 @default.
• W2047200410 cites W5029432 @default.
• W2047200410 cites W58588064 @default.
• W2047200410 cites W1977629567 @default.
• W2047200410 doi "https://doi.org/10.1002/(sici)1097-0282(19990415)49:5<385::aid-bip5>3.0.co;2-l" @default.
• W2047200410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11180047" @default.
• W2047200410 hasPublicationYear "1999" @default.
• W2047200410 type Work @default.
• W2047200410 sameAs 2047200410 @default.
• W2047200410 citedByCount "32" @default.
• W2047200410 countsByYear W20472004102012 @default.
• W2047200410 countsByYear W20472004102013 @default.
• W2047200410 countsByYear W20472004102015 @default.
• W2047200410 countsByYear W20472004102016 @default.
• W2047200410 countsByYear W20472004102017 @default.
• W2047200410 countsByYear W20472004102018 @default.
• W2047200410 countsByYear W20472004102020 @default.
• W2047200410 countsByYear W20472004102022 @default.
• W2047200410 crossrefType "journal-article" @default.
• W2047200410 hasAuthorship W2047200410A5032968167 @default.
• W2047200410 hasAuthorship W2047200410A5051021686 @default.
• W2047200410 hasAuthorship W2047200410A5067005790 @default.
• W2047200410 hasAuthorship W2047200410A5086939392 @default.
• W2047200410 hasConcept C113196181 @default.
• W2047200410 hasConcept C147789679 @default.
• W2047200410 hasConcept C159467904 @default.
• W2047200410 hasConcept C17525397 @default.
• W2047200410 hasConcept C178790620 @default.
• W2047200410 hasConcept C185592680 @default.
• W2047200410 hasConcept C199360897 @default.
• W2047200410 hasConcept C27703432 @default.
• W2047200410 hasConcept C2777027219 @default.
• W2047200410 hasConcept C2900643 @default.
• W2047200410 hasConcept C40250595 @default.
• W2047200410 hasConcept C41008148 @default.
• W2047200410 hasConcept C43617362 @default.
• W2047200410 hasConcept C521977710 @default.
• W2047200410 hasConcept C94786355 @default.
• W2047200410 hasConcept C95238685 @default.
• W2047200410 hasConceptScore W2047200410C113196181 @default.
• W2047200410 hasConceptScore W2047200410C147789679 @default.
• W2047200410 hasConceptScore W2047200410C159467904 @default.
• W2047200410 hasConceptScore W2047200410C17525397 @default.
• W2047200410 hasConceptScore W2047200410C178790620 @default.
• W2047200410 hasConceptScore W2047200410C185592680 @default.
• W2047200410 hasConceptScore W2047200410C199360897 @default.
• W2047200410 hasConceptScore W2047200410C27703432 @default.
• W2047200410 hasConceptScore W2047200410C2777027219 @default.
• W2047200410 hasConceptScore W2047200410C2900643 @default.
• W2047200410 hasConceptScore W2047200410C40250595 @default.
• W2047200410 hasConceptScore W2047200410C41008148 @default.
• W2047200410 hasConceptScore W2047200410C43617362 @default.
• W2047200410 hasConceptScore W2047200410C521977710 @default.
• W2047200410 hasConceptScore W2047200410C94786355 @default.
• W2047200410 hasConceptScore W2047200410C95238685 @default.
• W2047200410 hasIssue "5" @default.
• W2047200410 hasLocation W20472004101 @default.
• W2047200410 hasLocation W20472004102 @default.
• W2047200410 hasOpenAccess W2047200410 @default.

• next