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• W2047225913 endingPage "213" @default.
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• W2047225913 abstract "This report describes a method for the solubilization of a micro-particulate β-d-glucan. Insoluble glucan is dissolved in methyl sulfoxide and urea (8m) and partially phiosphorylated at 100°. The resulting water-soluble product is called glucan phosphate. The conversion rate is 70%, and the preparation is endotoxin free as determined by the Limulus lysate procedure. Glucan phosphate is composed of 34.66% C, 6.29% H, 42.83% O, and 2.23% P and has a repeating-unit empirical formula of (C6H10O5)7·PO3H2, indicating a phosphate group substitution on every seventh glucose subunit. Molecular-weight averages, polydispersity, and intrinsic viscosity were determined by aqueous high-performance size-exclusion chromatography (s.e.c.) with on-line, multi-angle laser light scattering (m.a.1.1.s) photometry and differential viscometry (d.v.). Two polymer peaks were resolved. Peak 1 (Mw = 3.57 × 106 daltons), represents ∼2% of the total polymers, while peak 2 (Mw = 1.10 × 105 daltons) comprises ∼98% of polymers. 13C- and 31P-n.m.r. spectroscopy confirmed the β-1,3 interchain linkage and the presence of a phosphate group. In solution, glucan phosphate polymers self-associate in a triple-helical arrangement. The ability to prepare a immunologically active, non-toxic, water-soluble β-d-glucan preparation will greatly enhance the clinical utility of this class of compounds." @default.
• W2047225913 created "2016-06-24" @default.
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• W2047225913 date "1991-10-01" @default.
• W2047225913 modified "2023-10-17" @default.
• W2047225913 title "A method for the solubilization of a (1→3)-β-d-glucan isolated from Saccharomyces cerevisiae" @default.
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• W2047225913 doi "https://doi.org/10.1016/0008-6215(91)89052-h" @default.
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