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- W2047252887 endingPage "177" @default.
- W2047252887 startingPage "157" @default.
- W2047252887 abstract "We report on the construction of maize minichromosomes using shuttle vectors harboring native centromeric segments, origins of replication, selectable marker genes, and telomeric repeats. These vectors were introduced into scutellar cells of maize immature embryos by microprojectile bombardment. Several independent transformation events were identified containing minichromosomes in addition to the normal diploid complement of 20 maize chromosomes. Immunostaining indicated that the minichromosomes recruited centromeric protein C, which is a specific component of the centromere/kinetochore complex. Minichromosomes were estimated to be 15-30 Mb in size based on cytological measurements. Fluorescent in situ hybridization (FISH) showed that minichromosomes contain the centromeric, telomeric, and exogenous unique marker sequences interspersed with maize retrotransposons. Minichromosomes were detected for at least a year in actively dividing callus cultures, providing evidence for their stability through numerous cell cycles. Plants were regenerated and minichromosomes were detected in root tips, providing confirmation of their normal replication and transmission during mitosis and through organogenesis. Assembly of maize artificial chromosomes may provide a tool to study centromere function and a foundation for developing new high capacity vectors for plant functional genomics and breeding." @default.
- W2047252887 created "2016-06-24" @default.
- W2047252887 creator A5019773129 @default.
- W2047252887 creator A5028501927 @default.
- W2047252887 creator A5028758259 @default.
- W2047252887 creator A5065771309 @default.
- W2047252887 creator A5078623877 @default.
- W2047252887 creator A5079799649 @default.
- W2047252887 creator A5083403093 @default.
- W2047252887 date "2008-11-18" @default.
- W2047252887 modified "2023-09-23" @default.
- W2047252887 title "Artificial chromosome formation in maize (Zea mays L.)" @default.
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