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• W2047321730 abstract "This work studies the expansion dynamics of molecules ejected from sample crystals in matrix-assisted laser desorption/ionization (MALDI) reaction. Measurements were made in two steps–sample desorption using a 355 nm pulsed laser beam and gas-phase postionization using a 266 nm laser at various delays. The estimated ion production yield is approximately 104 times better than that obtained using conventional MALDI methods. A model system of co-crystallized tryptophan (Trp) analyte and 2,4,6-trihydroxyacetophenone (THAP) matrix yielded overlapping time-of-arrival profiles. This result suggests that translational behaviours of gas-phase Trp and THAP are thermalized during expansion. The observed plume velocity depends strongly on the matrix and analyte composition: the most probable velocity is 214 m/s for pure Trp and 450 m/s for pure THAP. The incorporation of Trp into THAP makes both their velocities fall between the two extreme cases. Multiple half-space Maxwellian curves adequately describe the observed desorption velocity profile, implying that MALDI consists of multiple desorption components." @default.
• W2047321730 created "2016-06-24" @default.
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• W2047321730 date "2008-02-01" @default.
• W2047321730 modified "2023-09-25" @default.
• W2047321730 title "Desorption dynamics of neutral molecules in matrix-assisted laser desorption/ionization" @default.
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• W2047321730 doi "https://doi.org/10.1080/00268970701779671" @default.
• W2047321730 hasPublicationYear "2008" @default.
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