Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047334681> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2047334681 abstract "We describe a microplate-based, time-resolved fluorescence (TRF) spectrometer, able to detect multiple fluorescence lifetimes with acquisition times comparable to existing steady-state plate readers. The instrument combines high-energy pulsed laser sources (10-20 kHz repetition rate, 1-3 ns pulse width) with a photomultiplier and high-speed digitizer (1 GHz) to record a complete fluorescence decay waveform after each pulse. Pulse-averaged measurements of dyes in single wells yielded lifetimes comparable in accuracy and precision to single photon counting (SPC.) Lifetimes as short as 1 ns may be resolved by interleaving with an effective sample rate of 5 GHz. In a multiple-well format (96- or 384-well plates) the response of a dye to concentration gradients was easily observed, e.g., in quenching by acrylamide. The precision was sufficient to resolve mole fractions in mixtures of single-exponential dyes with an acquisition time of 0.5 s per well. In multiple-well experiments the variation in total measured fluorescence was comparable to steady-state instruments, while the precision in lifetime was better than 2%. These features will enable rapid, multiple-well, time-resolved FRET and fluorescence quenching experiments to determine changes of structural equilibria in solution, cells, and reconstituted systems." @default.
- W2047334681 created "2016-06-24" @default.
- W2047334681 creator A5006350427 @default.
- W2047334681 creator A5023379990 @default.
- W2047334681 creator A5024934620 @default.
- W2047334681 creator A5028487403 @default.
- W2047334681 creator A5077463067 @default.
- W2047334681 date "2013-01-01" @default.
- W2047334681 modified "2023-09-29" @default.
- W2047334681 title "A Microplate-Based Instrument for Resolving Fluorescence Lifetimes" @default.
- W2047334681 doi "https://doi.org/10.1016/j.bpj.2012.11.3771" @default.
- W2047334681 hasPublicationYear "2013" @default.
- W2047334681 type Work @default.
- W2047334681 sameAs 2047334681 @default.
- W2047334681 citedByCount "0" @default.
- W2047334681 crossrefType "journal-article" @default.
- W2047334681 hasAuthorship W2047334681A5006350427 @default.
- W2047334681 hasAuthorship W2047334681A5023379990 @default.
- W2047334681 hasAuthorship W2047334681A5024934620 @default.
- W2047334681 hasAuthorship W2047334681A5028487403 @default.
- W2047334681 hasAuthorship W2047334681A5077463067 @default.
- W2047334681 hasBestOaLocation W20473346811 @default.
- W2047334681 hasConcept C113196181 @default.
- W2047334681 hasConcept C113879476 @default.
- W2047334681 hasConcept C120665830 @default.
- W2047334681 hasConcept C121332964 @default.
- W2047334681 hasConcept C121745418 @default.
- W2047334681 hasConcept C159317903 @default.
- W2047334681 hasConcept C165801399 @default.
- W2047334681 hasConcept C185592680 @default.
- W2047334681 hasConcept C197424946 @default.
- W2047334681 hasConcept C2780167933 @default.
- W2047334681 hasConcept C2781402376 @default.
- W2047334681 hasConcept C33390570 @default.
- W2047334681 hasConcept C43617362 @default.
- W2047334681 hasConcept C520434653 @default.
- W2047334681 hasConcept C62520636 @default.
- W2047334681 hasConcept C91881484 @default.
- W2047334681 hasConcept C94915269 @default.
- W2047334681 hasConceptScore W2047334681C113196181 @default.
- W2047334681 hasConceptScore W2047334681C113879476 @default.
- W2047334681 hasConceptScore W2047334681C120665830 @default.
- W2047334681 hasConceptScore W2047334681C121332964 @default.
- W2047334681 hasConceptScore W2047334681C121745418 @default.
- W2047334681 hasConceptScore W2047334681C159317903 @default.
- W2047334681 hasConceptScore W2047334681C165801399 @default.
- W2047334681 hasConceptScore W2047334681C185592680 @default.
- W2047334681 hasConceptScore W2047334681C197424946 @default.
- W2047334681 hasConceptScore W2047334681C2780167933 @default.
- W2047334681 hasConceptScore W2047334681C2781402376 @default.
- W2047334681 hasConceptScore W2047334681C33390570 @default.
- W2047334681 hasConceptScore W2047334681C43617362 @default.
- W2047334681 hasConceptScore W2047334681C520434653 @default.
- W2047334681 hasConceptScore W2047334681C62520636 @default.
- W2047334681 hasConceptScore W2047334681C91881484 @default.
- W2047334681 hasConceptScore W2047334681C94915269 @default.
- W2047334681 hasLocation W20473346811 @default.
- W2047334681 hasOpenAccess W2047334681 @default.
- W2047334681 hasPrimaryLocation W20473346811 @default.
- W2047334681 hasRelatedWork W159976276 @default.
- W2047334681 hasRelatedWork W1903572583 @default.
- W2047334681 hasRelatedWork W1979103466 @default.
- W2047334681 hasRelatedWork W1986971495 @default.
- W2047334681 hasRelatedWork W1988516167 @default.
- W2047334681 hasRelatedWork W1998187290 @default.
- W2047334681 hasRelatedWork W2000873780 @default.
- W2047334681 hasRelatedWork W2004095371 @default.
- W2047334681 hasRelatedWork W2046163507 @default.
- W2047334681 hasRelatedWork W2081935408 @default.
- W2047334681 hasRelatedWork W2355656156 @default.
- W2047334681 hasRelatedWork W2361931951 @default.
- W2047334681 hasRelatedWork W2363332514 @default.
- W2047334681 hasRelatedWork W2383036843 @default.
- W2047334681 hasRelatedWork W2766454959 @default.
- W2047334681 hasRelatedWork W2792969679 @default.
- W2047334681 hasRelatedWork W2799784115 @default.
- W2047334681 hasRelatedWork W2914489172 @default.
- W2047334681 hasRelatedWork W2570381819 @default.
- W2047334681 hasRelatedWork W2593256666 @default.
- W2047334681 isParatext "false" @default.
- W2047334681 isRetracted "false" @default.
- W2047334681 magId "2047334681" @default.
- W2047334681 workType "article" @default.