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- W2047367038 abstract "The molecular characterization of the causal agent of diseases associated with several symptoms such as decline, yellowing,leaf roll and off-season growth in stone fruits made it possible to determine a common etiology, and the name ´European stone fruit yellows´ phytoplasma was proposed. Recently, the new taxonomical species description within the genus is 'Candidatus Phytoplasma prunorum'.A 2-yr survey was carried out in two different Prunus collections of the Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA) including European and Japanese plum genotypes of various species and several interspecific hybrids used as rootstocks. Both off-season growth in winter and decline were observed. In order to identify the phytoplasma suspected as a causal agent, two different PCR methods were applied to all inspected trees. The first method was a nested PCR with 16Sr X group-specific primers followed by RFLP analysis. Thesecond method was a direct PCR with specific primers for 'Ca P. prunorum' (Eca1/Eca2). In the most symptomatic treesthe presence of this phytoplasma was confirmed by at least one of the methods; negative results were obtained in asymptomatic trees. The nested PCR-RFLPs analysis was confirmed as a reliable method for routinary diagnosis rather than direct PCR." @default.
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- W2047367038 date "2009-06-01" @default.
- W2047367038 modified "2023-10-03" @default.
- W2047367038 title "Detection and identification of 'Candidatus Phytoplasma prunorum'in Prunus germplasm" @default.
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- W2047367038 doi "https://doi.org/10.5424/sjar/2009072-1503" @default.
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