Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047421343> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2047421343 endingPage "3051" @default.
- W2047421343 startingPage "3047" @default.
- W2047421343 abstract "A homologous in vitro transcription system was developed in which the cloned chicken vitellogenin II gene is faithfully transcribed by extracts prepared from chicken liver nuclei. The use of template deleted of its upstream region resulted in poor transcriptional efficiency, as did the use of extracts prepared from rooster liver, in which the gene is silent. The influence of individual cis elements was determined by transcription competition analysis. Oligonucleotides covering greater than 500 base pairs of the promoter region were used as competitor DNA in the in vitro reactions. Competition with an oligonucleotide covering part of the expression-specific DNase I hypersensitivity site B2, which contains a demethylation site, mCpG, at nucleotide position + 10, increased transcription of the gene, suggesting the binding of a repressor to this region. The enhancement of transcription was even more pronounced when the same oligonucleotide was methylated at the corresponding + 10 cytosine. Competition with oligonucleotides covering the TATA box, or the estrogen response element half-palindromic motif (GGTCA) at nucleotide positions -198 to -194, resulted in a large decrease in vitellogenin gene transcription, indicating that strongly activating factors bind to these regions. Competing oligonucleotides covering other GGTCA-containing motifs situated further upstream at nucleotide positions -292 to -288, -367 to -351, and -626 to -614 were increasingly less effective in inhibiting transcription. The results indicate that factors other than the estrogen receptor are involved in transcriptional activation of the vitellogenin II gene." @default.
- W2047421343 created "2016-06-24" @default.
- W2047421343 creator A5004385496 @default.
- W2047421343 creator A5062472371 @default.
- W2047421343 creator A5088778192 @default.
- W2047421343 date "1990-04-01" @default.
- W2047421343 modified "2023-10-16" @default.
- W2047421343 title "Positive and negative regulatory elements of chicken vitellogenin II gene characterized by in vitro transcription competition assays in a homologous system." @default.
- W2047421343 cites W1502922070 @default.
- W2047421343 cites W1540094397 @default.
- W2047421343 cites W1594016728 @default.
- W2047421343 cites W1964351745 @default.
- W2047421343 cites W1965258786 @default.
- W2047421343 cites W1971811011 @default.
- W2047421343 cites W1989555252 @default.
- W2047421343 cites W1991127901 @default.
- W2047421343 cites W1993156794 @default.
- W2047421343 cites W2003151593 @default.
- W2047421343 cites W2008128705 @default.
- W2047421343 cites W2018135163 @default.
- W2047421343 cites W2058415497 @default.
- W2047421343 cites W2063248559 @default.
- W2047421343 cites W2066441904 @default.
- W2047421343 cites W2073001950 @default.
- W2047421343 cites W2111990591 @default.
- W2047421343 cites W2117969708 @default.
- W2047421343 cites W2119859213 @default.
- W2047421343 cites W2128068280 @default.
- W2047421343 cites W2128635872 @default.
- W2047421343 cites W2134812217 @default.
- W2047421343 cites W2151071712 @default.
- W2047421343 cites W2159334258 @default.
- W2047421343 cites W2170236318 @default.
- W2047421343 cites W252338653 @default.
- W2047421343 cites W260259550 @default.
- W2047421343 doi "https://doi.org/10.1073/pnas.87.8.3047" @default.
- W2047421343 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/53831" @default.
- W2047421343 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2326265" @default.
- W2047421343 hasPublicationYear "1990" @default.
- W2047421343 type Work @default.
- W2047421343 sameAs 2047421343 @default.
- W2047421343 citedByCount "27" @default.
- W2047421343 crossrefType "journal-article" @default.
- W2047421343 hasAuthorship W2047421343A5004385496 @default.
- W2047421343 hasAuthorship W2047421343A5062472371 @default.
- W2047421343 hasAuthorship W2047421343A5088778192 @default.
- W2047421343 hasBestOaLocation W20474213431 @default.
- W2047421343 hasConcept C101762097 @default.
- W2047421343 hasConcept C104317684 @default.
- W2047421343 hasConcept C129312508 @default.
- W2047421343 hasConcept C132376346 @default.
- W2047421343 hasConcept C138885662 @default.
- W2047421343 hasConcept C150194340 @default.
- W2047421343 hasConcept C153911025 @default.
- W2047421343 hasConcept C179926584 @default.
- W2047421343 hasConcept C41895202 @default.
- W2047421343 hasConcept C44667518 @default.
- W2047421343 hasConcept C54355233 @default.
- W2047421343 hasConcept C76557 @default.
- W2047421343 hasConcept C86803240 @default.
- W2047421343 hasConcept C98108389 @default.
- W2047421343 hasConceptScore W2047421343C101762097 @default.
- W2047421343 hasConceptScore W2047421343C104317684 @default.
- W2047421343 hasConceptScore W2047421343C129312508 @default.
- W2047421343 hasConceptScore W2047421343C132376346 @default.
- W2047421343 hasConceptScore W2047421343C138885662 @default.
- W2047421343 hasConceptScore W2047421343C150194340 @default.
- W2047421343 hasConceptScore W2047421343C153911025 @default.
- W2047421343 hasConceptScore W2047421343C179926584 @default.
- W2047421343 hasConceptScore W2047421343C41895202 @default.
- W2047421343 hasConceptScore W2047421343C44667518 @default.
- W2047421343 hasConceptScore W2047421343C54355233 @default.
- W2047421343 hasConceptScore W2047421343C76557 @default.
- W2047421343 hasConceptScore W2047421343C86803240 @default.
- W2047421343 hasConceptScore W2047421343C98108389 @default.
- W2047421343 hasIssue "8" @default.
- W2047421343 hasLocation W20474213431 @default.
- W2047421343 hasLocation W20474213432 @default.
- W2047421343 hasLocation W20474213433 @default.
- W2047421343 hasLocation W20474213434 @default.
- W2047421343 hasOpenAccess W2047421343 @default.
- W2047421343 hasPrimaryLocation W20474213431 @default.
- W2047421343 hasRelatedWork W1988402913 @default.
- W2047421343 hasRelatedWork W2009966535 @default.
- W2047421343 hasRelatedWork W2074386014 @default.
- W2047421343 hasRelatedWork W2075341877 @default.
- W2047421343 hasRelatedWork W2111182449 @default.
- W2047421343 hasRelatedWork W2121924440 @default.
- W2047421343 hasRelatedWork W2148922163 @default.
- W2047421343 hasRelatedWork W2179812999 @default.
- W2047421343 hasRelatedWork W2355447014 @default.
- W2047421343 hasRelatedWork W2896653760 @default.
- W2047421343 hasVolume "87" @default.
- W2047421343 isParatext "false" @default.
- W2047421343 isRetracted "false" @default.
- W2047421343 magId "2047421343" @default.
- W2047421343 workType "article" @default.