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- W2047500522 abstract "We describe the first systematic evaluation of reference genes for use in real-time quantitative polymerase chain reaction (qPCR) for water deficit stress studies in the citrus rootstock “Swingle” citrumelo. The expression levels of seven reference genes—cyclophilin (CYP), cathepsin (CtP), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1α (EF1α), β-tubulin (TUB), and ADP ribosylation factor (ADP)—during drought stress were tested using geNorm and NormFinder programs. Results from four experimental conditions indicated that EF1α and ADP were the most stable reference genes. Relative expression levels of Δ1-pyrroline-5-carboxylate synthetase (P5CS) was used for reference gene validation." @default.
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- W2047500522 date "2010-07-01" @default.
- W2047500522 modified "2023-10-02" @default.
- W2047500522 title "Reference gene selection for real-time quantitative polymerase chain reaction normalization in “Swingle” citrumelo under drought stress" @default.
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- W2047500522 doi "https://doi.org/10.1016/j.ab.2010.03.038" @default.
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