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• W2047599497 abstract "3,4-Dihydroxy l-phenylalanine (l-DOPA) is considered a potent drug for the treatment of Parkinson disease. Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of l-DOPA. Screenings of critical components were completed by using a Plackett-Burman design, while further optimization was carried out using the Box-Behnken design. The optimized factor levels predicted by the model were pH 6.1, 1.659 g L(-1) yeast extract, 1.491 g L(-1)l-tyrosine and 0.0290 g L(-1) CuSO4. The predicted yield of l-DOPA with these levels was 1.319 g L(-1), while actual yield obtained was 1.273 g L(-1). The statistical analysis revealed that model is significant with F value 19.55 and R(2) value 0.9514. This process resulted in a 3.594-fold increase in the yield of l-DOPA. l-DOPA was confirmed by HPTLC and HPLC analysis. Thus, Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of l-DOPA." @default.
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• W2047599497 date "2014-05-07" @default.
• W2047599497 modified "2023-09-23" @default.
• W2047599497 title "Evaluation of Various Factors Affecting Bioconversion of l-Tyrosine to l-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology" @default.
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• W2047599497 doi "https://doi.org/10.1007/s13659-014-0017-3" @default.
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