FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047607524> ?p ?o ?g. }

• W2047607524 endingPage "397" @default.
• W2047607524 startingPage "397" @default.
• W2047607524 abstract "Allergen extracts from the house dust mite (HDM, Dermatophagoides pteronyssinus) are widely utilized for diagnosis and treatment of allergic diseases. It is known that allergen extracts degrade and lose potency when stored over time.This study aimed to determine the optimal conditions for stability of allergen extracts. This study was undertaken to investigate the optimal storage conditions for HDM extracts, the effects of adding 0.03% human serum albumin (HSA) and 50% glycerol were evaluated at -20℃, 4℃, and room temperature (RT). Changes in protein and group 1 major allergen (Der p 1) concentration, as well as allergenicity were measured over a 1 year period using the Bradford assay, two-site ELISA, and ELISA inhibition.Protein concentrations decreased by 86%, 51%, and 6% at RT, 4℃, and -20℃, respectively, when stored in distilled water. Overall allergenicity remained high (89.9%) when the extracts was reconstituted in 50% glycerol solution, and was 93.1% when reconstituted in 50% glycerol and 0.03% HSA at RT. Allergenicity was decreased to 36.6% and 33.3%, however, reconstitution in DW or 0.03% HSA solution at RT, respectively. Allergenicity was remained high as 92.0%-97.0% when stored at 4℃ regardless of the buffer conditions.Storage temperature is the most important factor in preserving allergenicity of HDM extracts, which is ideal at 4℃. The addition of 50% glycerol to the storage buffer was also found to play an important role in increasing the shelf-life of HDM extracts at RT." @default.
• W2047607524 created "2016-06-24" @default.
• W2047607524 creator A5000693926 @default.
• W2047607524 creator A5016998114 @default.
• W2047607524 creator A5021400296 @default.
• W2047607524 creator A5023696368 @default.
• W2047607524 creator A5025400440 @default.
• W2047607524 creator A5049646984 @default.
• W2047607524 creator A5078229156 @default.
• W2047607524 date "2013-01-01" @default.
• W2047607524 modified "2023-10-16" @default.
• W2047607524 title "The Effects of Storage Conditions on the Stability of House Dust Mite Extracts" @default.
• W2047607524 cites W1990332463 @default.
• W2047607524 cites W1992708426 @default.
• W2047607524 cites W1994534886 @default.
• W2047607524 cites W2001024712 @default.
• W2047607524 cites W2001951274 @default.
• W2047607524 cites W2003163020 @default.
• W2047607524 cites W2027650550 @default.
• W2047607524 cites W2063255619 @default.
• W2047607524 cites W2065689359 @default.
• W2047607524 cites W2068325540 @default.
• W2047607524 cites W2086245537 @default.
• W2047607524 cites W2111094023 @default.
• W2047607524 cites W2144443144 @default.
• W2047607524 cites W2159157688 @default.
• W2047607524 cites W2164850324 @default.
• W2047607524 cites W2468151660 @default.
• W2047607524 doi "https://doi.org/10.4168/aair.2013.5.6.397" @default.
• W2047607524 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3810547" @default.
• W2047607524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24179687" @default.
• W2047607524 hasPublicationYear "2013" @default.
• W2047607524 type Work @default.
• W2047607524 sameAs 2047607524 @default.
• W2047607524 citedByCount "12" @default.
• W2047607524 countsByYear W20476075242014 @default.
• W2047607524 countsByYear W20476075242015 @default.
• W2047607524 countsByYear W20476075242016 @default.
• W2047607524 countsByYear W20476075242017 @default.
• W2047607524 countsByYear W20476075242018 @default.
• W2047607524 countsByYear W20476075242019 @default.
• W2047607524 countsByYear W20476075242020 @default.
• W2047607524 countsByYear W20476075242021 @default.
• W2047607524 crossrefType "journal-article" @default.
• W2047607524 hasAuthorship W2047607524A5000693926 @default.
• W2047607524 hasAuthorship W2047607524A5016998114 @default.
• W2047607524 hasAuthorship W2047607524A5021400296 @default.
• W2047607524 hasAuthorship W2047607524A5023696368 @default.
• W2047607524 hasAuthorship W2047607524A5025400440 @default.
• W2047607524 hasAuthorship W2047607524A5049646984 @default.
• W2047607524 hasAuthorship W2047607524A5078229156 @default.
• W2047607524 hasBestOaLocation W20476075242 @default.
• W2047607524 hasConcept C115930662 @default.
• W2047607524 hasConcept C185592680 @default.
• W2047607524 hasConcept C202751555 @default.
• W2047607524 hasConcept C203014093 @default.
• W2047607524 hasConcept C207480886 @default.
• W2047607524 hasConcept C2778166374 @default.
• W2047607524 hasConcept C2779429693 @default.
• W2047607524 hasConcept C2780510475 @default.
• W2047607524 hasConcept C2780881558 @default.
• W2047607524 hasConcept C31903555 @default.
• W2047607524 hasConcept C43617362 @default.
• W2047607524 hasConcept C55493867 @default.
• W2047607524 hasConcept C57992300 @default.
• W2047607524 hasConcept C86803240 @default.
• W2047607524 hasConceptScore W2047607524C115930662 @default.
• W2047607524 hasConceptScore W2047607524C185592680 @default.
• W2047607524 hasConceptScore W2047607524C202751555 @default.
• W2047607524 hasConceptScore W2047607524C203014093 @default.
• W2047607524 hasConceptScore W2047607524C207480886 @default.
• W2047607524 hasConceptScore W2047607524C2778166374 @default.
• W2047607524 hasConceptScore W2047607524C2779429693 @default.
• W2047607524 hasConceptScore W2047607524C2780510475 @default.
• W2047607524 hasConceptScore W2047607524C2780881558 @default.
• W2047607524 hasConceptScore W2047607524C31903555 @default.
• W2047607524 hasConceptScore W2047607524C43617362 @default.
• W2047607524 hasConceptScore W2047607524C55493867 @default.
• W2047607524 hasConceptScore W2047607524C57992300 @default.
• W2047607524 hasConceptScore W2047607524C86803240 @default.
• W2047607524 hasFunder F4320322034 @default.
• W2047607524 hasIssue "6" @default.
• W2047607524 hasLocation W20476075241 @default.
• W2047607524 hasLocation W20476075242 @default.
• W2047607524 hasLocation W20476075243 @default.
• W2047607524 hasLocation W20476075244 @default.
• W2047607524 hasLocation W20476075245 @default.
• W2047607524 hasOpenAccess W2047607524 @default.
• W2047607524 hasPrimaryLocation W20476075241 @default.
• W2047607524 hasRelatedWork W134903949 @default.
• W2047607524 hasRelatedWork W2047607524 @default.
• W2047607524 hasRelatedWork W2141856163 @default.
• W2047607524 hasRelatedWork W2154920466 @default.
• W2047607524 hasRelatedWork W2169078421 @default.
• W2047607524 hasRelatedWork W2282954330 @default.
• W2047607524 hasRelatedWork W2407921974 @default.
• W2047607524 hasRelatedWork W2790029715 @default.
• W2047607524 hasRelatedWork W2926348909 @default.

• next