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• W2047610620 abstract "A synthetic Bacillus thuringiensis (Bt) cry1C gene under the control of the 35S CaMV promoter was introduced into cauliflower (Brassica oleracea var. botrytis) by Agrobacterium tumefaciens- mediated transformation with hygromycin selection. A total of 35 transgenic plants were regenerated from six cultivars (Freemont, Candid Charm, Snow Crown, Cumberland, Majestic, and Cashmere) with average transformation efficiency of 0.3% to 6.4%. All the hygromycin-resistant transformants also carried the Bt gene, as shown by PCR with primers specific to the cry1C gene. ELISA analysis showed that the levels of Cry1C protein in independent transformants varied widely, from 0 to 0.2% of total soluble protein. The majority of the plants (61%) produced a high level of Cry1C protein (> 1000 ng mg_1 proteins). Insect bioassays demonstrated that plants producing Cry1C protein effectively controlled larvae of diamondback moths (Plutella xylostella), including ones resistant to Cry1A protein, as well as larvae of cabbage loopers (Trichoplusia ni). These cry1C cauliflower plants will be useful for further studies, especially in comparisons with cauliflower plants carrying the same cry1c gene under control of a light-inducible promoter." @default.
• W2047610620 created "2016-06-24" @default.
• W2047610620 creator A5061078545 @default.
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• W2047610620 date "2003-04-01" @default.
• W2047610620 modified "2023-10-18" @default.
• W2047610620 title "Cauliflower Plants Expressing a cry1C Transgene Control Larvae of Diamondback Moths Resistant or Susceptible to Cry1A, and Cabbage Loopers" @default.
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• W2047610620 doi "https://doi.org/10.1300/j153v05n02_06" @default.
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