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- W2047675352 abstract "Abstract We have studied the expression of the 48K host phosphoprotein after primary infection of BALB/c 3T3 mouse cells with simian virus 40, using a monospecific antiserum against the purified 48K protein. The 48K host phosphoprotein, previously shown to complex with SV40 large T Ag, is shown to be expressed in the nuclei of BALB/c 3T3 cells 18–24 hr after primary infection by SV40, at the same time large T Ag is expressed. Expression of the 48K protein shows the same distribution and multiplicity dependence as that of large T Ag. We were able, by comparing percentages of cells stained with α94K, or α48K, or both antisera together, to show all cells expressing large T Ag and only those cells expressing large T Ag also express the 48K protein. The SV40 A locus governs the expression of the host 48K phosphoprotein, while deletions in the region 0.54 to 0.58 have no effect, suggesting it is large and not small T Ag which governs the expression of the host 48K phosphoprotein. The adenovirus-SV40 hybrid virus, AD2D2, also causes the expression of the host 48K protein, while adenovirus 2 alone does not, also indicating that it is the region 0.54-0.17 map units of the SV40 genome which governs the expression of the 48K protein." @default.
- W2047675352 created "2016-06-24" @default.
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- W2047675352 date "1980-04-01" @default.
- W2047675352 modified "2023-09-23" @default.
- W2047675352 title "Coordinate expression of the 48K host nuclear phosphoprotein and SV40 T Ag upon primary infection of mouse cells" @default.
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- W2047675352 doi "https://doi.org/10.1016/0042-6822(80)90112-9" @default.
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