Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047698813> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2047698813 endingPage "52" @default.
- W2047698813 startingPage "47" @default.
- W2047698813 abstract "Snake venom phosphodiesterase, bovine spleen phosphodiesterase and polynucleotide phosphorylase are currently used for sequence analysis of oligoribonucleotides. These exonucleases remove mononucleotide residues in a sequential order from either the 3’or 5’-end of an oligonucleotide. The nucleotide sequence of a given oligonucleotide is then derived by analyzing the partially degraded oligonucleotides which appear in the course of the reaction (cf. [l-3] ). These procedures, while very useful for analyzing labeled oligonucleotides, are inadequate when only trace amounts of non-labeled material are available. In the present report we describe a novel and sensitive method for sequence analysis of non-labeled oligoribonucleotides. The method is based on the property of polynucleotide phosphorylase to phosphorolyze short oligonucleotides from the 3’-end in a stepwise fashion by a nonprocessive mechanism [4-61. The final products of the reaction consist of a mixture of nucleoside diphosphates (NDP’s) and a limit oligonucleotide that is not further degraded by the enzyme. Depending on its base composition, this oligonucleotide is a dior trinucleotide. By following the order in which the released NDP’s appear during the phosphorolysis of a given oligonucleotide, it is possible to determine the nucleotide sequence from the 3’-end up to 2-3 residues from the 5’-terminus. The high sensitivity of this method is achieved by including 32Pi in the phosphorolysis medium. Upon digestion, the label is incorporated in the P-phosphate moieties of the generated NDP’s which can be easily" @default.
- W2047698813 created "2016-06-24" @default.
- W2047698813 creator A5053719429 @default.
- W2047698813 creator A5061498066 @default.
- W2047698813 creator A5070239030 @default.
- W2047698813 date "1973-04-01" @default.
- W2047698813 modified "2023-10-13" @default.
- W2047698813 title "Stepwise phosphorolysis with polynucleotide phosphorylase: A novel method for sequence analysis of oligoribonucleotides" @default.
- W2047698813 cites W1506151980 @default.
- W2047698813 cites W1515606077 @default.
- W2047698813 cites W1518804869 @default.
- W2047698813 cites W1568582513 @default.
- W2047698813 cites W1569670216 @default.
- W2047698813 cites W1832598496 @default.
- W2047698813 cites W2017855237 @default.
- W2047698813 cites W2022214565 @default.
- W2047698813 cites W2033162194 @default.
- W2047698813 cites W2048284900 @default.
- W2047698813 cites W2083149513 @default.
- W2047698813 cites W51261522 @default.
- W2047698813 doi "https://doi.org/10.1016/0014-5793(73)80071-7" @default.
- W2047698813 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4575634" @default.
- W2047698813 hasPublicationYear "1973" @default.
- W2047698813 type Work @default.
- W2047698813 sameAs 2047698813 @default.
- W2047698813 citedByCount "12" @default.
- W2047698813 crossrefType "journal-article" @default.
- W2047698813 hasAuthorship W2047698813A5053719429 @default.
- W2047698813 hasAuthorship W2047698813A5061498066 @default.
- W2047698813 hasAuthorship W2047698813A5070239030 @default.
- W2047698813 hasConcept C181199279 @default.
- W2047698813 hasConcept C185592680 @default.
- W2047698813 hasConcept C2776476023 @default.
- W2047698813 hasConcept C2777648924 @default.
- W2047698813 hasConcept C2778112365 @default.
- W2047698813 hasConcept C2779478826 @default.
- W2047698813 hasConcept C55493867 @default.
- W2047698813 hasConcept C71615608 @default.
- W2047698813 hasConceptScore W2047698813C181199279 @default.
- W2047698813 hasConceptScore W2047698813C185592680 @default.
- W2047698813 hasConceptScore W2047698813C2776476023 @default.
- W2047698813 hasConceptScore W2047698813C2777648924 @default.
- W2047698813 hasConceptScore W2047698813C2778112365 @default.
- W2047698813 hasConceptScore W2047698813C2779478826 @default.
- W2047698813 hasConceptScore W2047698813C55493867 @default.
- W2047698813 hasConceptScore W2047698813C71615608 @default.
- W2047698813 hasIssue "1" @default.
- W2047698813 hasLocation W20476988131 @default.
- W2047698813 hasLocation W20476988132 @default.
- W2047698813 hasOpenAccess W2047698813 @default.
- W2047698813 hasPrimaryLocation W20476988131 @default.
- W2047698813 hasRelatedWork W1515606077 @default.
- W2047698813 hasRelatedWork W1526321695 @default.
- W2047698813 hasRelatedWork W1550196709 @default.
- W2047698813 hasRelatedWork W1568582513 @default.
- W2047698813 hasRelatedWork W1975498316 @default.
- W2047698813 hasRelatedWork W1977525708 @default.
- W2047698813 hasRelatedWork W2038743954 @default.
- W2047698813 hasRelatedWork W2081741878 @default.
- W2047698813 hasRelatedWork W2097698204 @default.
- W2047698813 hasRelatedWork W268908615 @default.
- W2047698813 hasVolume "31" @default.
- W2047698813 isParatext "false" @default.
- W2047698813 isRetracted "false" @default.
- W2047698813 magId "2047698813" @default.
- W2047698813 workType "article" @default.