FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047715774> ?p ?o ?g. }

• W2047715774 endingPage "1588" @default.
• W2047715774 startingPage "1579" @default.
• W2047715774 abstract "1 We investigated how microsomal cytochrome P450 mono-oxygenase (Cyp450 MO) is regulated in cultured porcine aortic endothelial cells. The hypothesis that a Cyp450 MO-derived metabolite links Ca2+ store depletion and Ca2+ entry was studied further. 2 Microsomal Cyp450 MO was monitored fluorometrically by dealkylation of 1-ethoxypyrene-3,6,8-tris-(dimethyl-sulphonamide; EPSA) in saponin permeabilized cells or in subcellular compartments. Endothelial Ca2+ signalling was measured by a standard fura-2 technique, membrane potential was determined with the potential-sensitive fluorescence dye, bis-(1,3-dibutylbarbituric acid) pentamethine oxonol (DiBAC4(5)) and tyrosine kinase was quantified by measuring the phosphorylation of a immobilized substrate with a horseradish peroxidase labelled phosphotyrosine specific antibody. 3 Depletion of cellular Ca2+ pools with inositol 1,4,5-trisphosphate (IP3), thapsigargin or cyclopiazonic acid activated microsomal Cyp450 MO. Similar to direct Ca2+ store depletion, chelating of intra-microsomal Ca2+ with oxalate stimulated Cyp450 MO activity, while changing cytosolic free Ca2+ failed to influence Cyp450 MO activity. These data indicate that microsomal Cyp450 MO is activated by depletion of IP3-sensitive stores. 4 Besides the common cytochrome P450 inhibitors, econazole, proadifen and miconazole, thiopentone sodium and methohexitone inhibited Cyp450 MO in a concentration-dependent manner. The physiological substrate of Cyp450 MO, arachidonic acid, inhibited EPSA dealkylation. In contrast to most other cytochrome P450 inhibitors used in this study, thiopentone sodium did not directly interfere with Ca2+ entry pathways, membrane hyperpolarization due to K+ channel activation or tyrosine kinase activity. 5 Inhibition of Cyp450 MO by thiopentone sodium diminished Ca2+/Mn2+ entry to Ca2+ store depletion by 43%, while it did not interfere with intracellular Ca2+ release by IP3 or thapsigargin. 6 Cyp450 MO inhibition with thiopentone sodium diminished autacoid-induced membrane hyperpolarization. 7 Induction of Cyp450 MO with dexamethasone/clofibrate for 72 h yielded increases in thapsigargin-induced Cyp450 MO activity (by 35%), Ca2+/Mn2+ entry (by 105%) and membrane hyperpolarization (by 40%). 8 The Cyp450 MO-derived compounds, 11,12 and 5,6-epoxyeicosatrienoic acids (EETs) yielded membrane hyperpolarization, insensitive to thiopentone sodium. 9 These data demonstrate that endothelial Cyp450 MO is activated by Ca2+ store depletion and Cyp450 MO produced compounds that hyperpolarize endothelial cells. 10 The data presented and our previous findings indicate that Cyp450 MO plays a crucial role in the regulation of store-operated Ca2+ influx. We propose that Cyp450 MO-derived EETs constitute a signal for Ca2+ entry activation and increase the driving force for Ca2+ entry by membrane hyperpolarization in porcine aortic endothelial cells. British Journal of Pharmacology (1997) 121, 1579–1588; doi:10.1038/sj.bjp.0701304" @default.
• W2047715774 created "2016-06-24" @default.
• W2047715774 creator A5021827648 @default.
• W2047715774 creator A5053111848 @default.
• W2047715774 creator A5065384976 @default.
• W2047715774 date "1997-08-01" @default.
• W2047715774 modified "2023-10-14" @default.
• W2047715774 title "Activation of microsomal cytochrome P450 mono-oxygenase by Ca²⁺store depletion and its contribution to Ca²⁺entry in porcine aortic endothelial cells" @default.
• W2047715774 cites W143202472 @default.
• W2047715774 cites W1537305348 @default.
• W2047715774 cites W1601900247 @default.
• W2047715774 cites W1605909812 @default.
• W2047715774 cites W1663123254 @default.
• W2047715774 cites W1857056367 @default.
• W2047715774 cites W1874327812 @default.
• W2047715774 cites W1968243333 @default.
• W2047715774 cites W1969455975 @default.
• W2047715774 cites W1981279166 @default.
• W2047715774 cites W1983545392 @default.
• W2047715774 cites W1990251248 @default.
• W2047715774 cites W1990930369 @default.
• W2047715774 cites W1993404169 @default.
• W2047715774 cites W1997702857 @default.
• W2047715774 cites W1998606603 @default.
• W2047715774 cites W1999110844 @default.
• W2047715774 cites W2002744696 @default.
• W2047715774 cites W2003907220 @default.
• W2047715774 cites W2009653931 @default.
• W2047715774 cites W2047639449 @default.
• W2047715774 cites W2064091371 @default.
• W2047715774 cites W2069846805 @default.
• W2047715774 cites W2074410830 @default.
• W2047715774 cites W2077785024 @default.
• W2047715774 cites W2102681387 @default.
• W2047715774 cites W2104413959 @default.
• W2047715774 cites W2113722141 @default.
• W2047715774 cites W2144486173 @default.
• W2047715774 cites W2153999923 @default.
• W2047715774 cites W2237551810 @default.
• W2047715774 cites W2301092763 @default.
• W2047715774 cites W2343332962 @default.
• W2047715774 cites W2799503982 @default.
• W2047715774 cites W61604311 @default.
• W2047715774 doi "https://doi.org/10.1038/sj.bjp.0701304" @default.
• W2047715774 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1564862" @default.
• W2047715774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9283690" @default.
• W2047715774 hasPublicationYear "1997" @default.
• W2047715774 type Work @default.
• W2047715774 sameAs 2047715774 @default.
• W2047715774 citedByCount "76" @default.
• W2047715774 countsByYear W20477157742013 @default.
• W2047715774 countsByYear W20477157742015 @default.
• W2047715774 countsByYear W20477157742018 @default.
• W2047715774 crossrefType "journal-article" @default.
• W2047715774 hasAuthorship W2047715774A5021827648 @default.
• W2047715774 hasAuthorship W2047715774A5053111848 @default.
• W2047715774 hasAuthorship W2047715774A5065384976 @default.
• W2047715774 hasBestOaLocation W20477157741 @default.
• W2047715774 hasConcept C158617107 @default.
• W2047715774 hasConcept C181199279 @default.
• W2047715774 hasConcept C185592680 @default.
• W2047715774 hasConcept C2777146706 @default.
• W2047715774 hasConcept C2779996123 @default.
• W2047715774 hasConcept C55493867 @default.
• W2047715774 hasConcept C87644729 @default.
• W2047715774 hasConceptScore W2047715774C158617107 @default.
• W2047715774 hasConceptScore W2047715774C181199279 @default.
• W2047715774 hasConceptScore W2047715774C185592680 @default.
• W2047715774 hasConceptScore W2047715774C2777146706 @default.
• W2047715774 hasConceptScore W2047715774C2779996123 @default.
• W2047715774 hasConceptScore W2047715774C55493867 @default.
• W2047715774 hasConceptScore W2047715774C87644729 @default.
• W2047715774 hasIssue "8" @default.
• W2047715774 hasLocation W20477157741 @default.
• W2047715774 hasLocation W20477157742 @default.
• W2047715774 hasLocation W20477157743 @default.
• W2047715774 hasLocation W20477157744 @default.
• W2047715774 hasOpenAccess W2047715774 @default.
• W2047715774 hasPrimaryLocation W20477157741 @default.
• W2047715774 hasRelatedWork W1505796222 @default.
• W2047715774 hasRelatedWork W1988774139 @default.
• W2047715774 hasRelatedWork W2010886092 @default.
• W2047715774 hasRelatedWork W2014631886 @default.
• W2047715774 hasRelatedWork W2035307965 @default.
• W2047715774 hasRelatedWork W2067286281 @default.
• W2047715774 hasRelatedWork W2070661410 @default.
• W2047715774 hasRelatedWork W2332955794 @default.
• W2047715774 hasRelatedWork W2739625907 @default.
• W2047715774 hasRelatedWork W3105414812 @default.
• W2047715774 hasVolume "121" @default.
• W2047715774 isParatext "false" @default.
• W2047715774 isRetracted "false" @default.
• W2047715774 magId "2047715774" @default.
• W2047715774 workType "article" @default.