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• W2047726095 abstract "Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA duplex through a transient enzyme-bridged double-stranded break in another segment. The ATP-dependent passage reaction has previously been demonstrated at the single-molecule level, showing apparent processivity at saturating ATP. Here we directly observed the strand passage by human topoisomerase IIα, after winding a pair of fluorescently stained DNA molecules with optical tweezers for 30 turns into an X-shaped braid. On average 0.51 ± 0.33 µm (11 ± 6 turns) of a braid was unlinked in a burst of reactions taking 8 ± 4 s, the unlinked length being essentially independent of the enzyme concentration between 0.25-37 pM. The time elapsed before the start of processive unlinking decreased with the enzyme concentration, being ~100 s at 3.7 pM. These results are consistent with a scenario where the enzyme binds to one DNA for a period of ~10 s, waiting for multiple diffusional encounters with the other DNA to transport it across the break ~10 times, and then dissociates from the binding site without waiting for the exhaustion of transportable DNA segments." @default.
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• W2047726095 date "2012-04-09" @default.
• W2047726095 modified "2023-10-05" @default.
• W2047726095 title "Direct Observation of Strand Passage by DNA-Topoisomerase and Its Limited Processivity" @default.
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• W2047726095 doi "https://doi.org/10.1371/journal.pone.0034920" @default.
• W2047726095 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3322154" @default.
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