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- W2047729307 abstract "The thermal stability of the mRNA-aminoacyl-tRNA-ribosome complex and each of its individual components was examined. A temperature-dependent, reversible dissociation of the mRNA-tRNA-ribosome complex has been demonstrated. By analogy with polynucleotide melting curves, the dissociation of the mRNA-tRNA-ribosome complex is considered as a pseudo-phase transition characterized by a melting temperature, Tm. The nature of all three components of the complex affects the Tm values. The following factors in the mRNA component were demonstrated to influence the Tm of the complex: composition of the codon, the non-codon bases, the chain length, and the position of the terminal phosphate group. A comparison of Tm values of the complex for tRNA molecules containing only A–U base pairs in their codon-anticodons indicates that regions of the tRNA molecules outside the anticodon may influence the stability of the complex. The source of the ribosomes modifies the Tm of the complex, while the method of preparation of the ribosomes appears to have no effect. The mRNA (trinucleotide)-tRNA-ribosome complex is almost 40 °C more stable than the corresponding trinucleotide-polynucleotide complex in the same buffer." @default.
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- W2047729307 date "1968-03-01" @default.
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- W2047729307 title "Stability of the messenger RNA-transfer RNA-ribosome complex" @default.
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- W2047729307 doi "https://doi.org/10.1016/0022-2836(68)90341-0" @default.
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