FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2047734512> ?p ?o ?g. }

• W2047734512 endingPage "2568" @default.
• W2047734512 startingPage "2558" @default.
• W2047734512 abstract "Calcium- and integrin-binding protein (CIB) is a novel member of the helix-loop-helix family of regulatory calcium-binding proteins which likely has a specific function in hemostasis through its interaction with platelet integrin alphaIIbbeta(3). The significant amino acid sequence homology between CIB and other regulatory calcium-binding proteins such as calmodulin, calcineurin B, and recoverin suggests that CIB may undergo a calcium-induced conformational change; however, the mechanism of calcium binding and the details of a structural change have not yet been investigated. Consequently, we have performed a variety of spectroscopic and microcalorimetric studies of CIB to determine its calcium binding characteristics, and the subsequent conformational changes that occur. Furthermore, we provide the first evidence for magnesium binding to CIB and determine the structural consequences of this interaction. Our results indicate that in the absence of any bound metal ions, apo-CIB adopts a folded yet highly flexible molten globule-like structure. Both calcium and magnesium binding induce conformational changes which stabilize both the secondary and tertiary structure of CIB, resulting in considerable increases in the thermal stability of the proteins. CIB was found to bind two Ca(2+) ions in a sequential manner with dissociation constants (K(d)) near 0.54 and 1.9 microM for sites EF-4 and EF-3, respectively. In contrast, CIB bound only one Mg(2+) ion to EF-3 with a K(d) near 120 microM. Together, our results suggest that CIB may exist in multiple structural and metal ion-bound states in vivo which may play a role in its regulation of target proteins such as platelet integrin." @default.
• W2047734512 created "2016-06-24" @default.
• W2047734512 creator A5030359755 @default.
• W2047734512 creator A5042050825 @default.
• W2047734512 creator A5042190192 @default.
• W2047734512 creator A5066066688 @default.
• W2047734512 date "2004-02-13" @default.
• W2047734512 modified "2023-10-03" @default.
• W2047734512 title "Metal Ion Binding Properties and Conformational States of Calcium- and Integrin-Binding Protein" @default.
• W2047734512 cites W1523171733 @default.
• W2047734512 cites W1524268590 @default.
• W2047734512 cites W1562088891 @default.
• W2047734512 cites W1562947759 @default.
• W2047734512 cites W1565248232 @default.
• W2047734512 cites W1866478874 @default.
• W2047734512 cites W1973092758 @default.
• W2047734512 cites W1979191356 @default.
• W2047734512 cites W1985523070 @default.
• W2047734512 cites W2050552445 @default.
• W2047734512 cites W2066114323 @default.
• W2047734512 cites W2069602336 @default.
• W2047734512 cites W2097813999 @default.
• W2047734512 cites W2110288394 @default.
• W2047734512 cites W2110906301 @default.
• W2047734512 cites W2124696627 @default.
• W2047734512 cites W2172196619 @default.
• W2047734512 cites W2209250776 @default.
• W2047734512 cites W4234174093 @default.
• W2047734512 doi "https://doi.org/10.1021/bi035432b" @default.
• W2047734512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14992593" @default.
• W2047734512 hasPublicationYear "2004" @default.
• W2047734512 type Work @default.
• W2047734512 sameAs 2047734512 @default.
• W2047734512 citedByCount "74" @default.
• W2047734512 countsByYear W20477345122012 @default.
• W2047734512 countsByYear W20477345122013 @default.
• W2047734512 countsByYear W20477345122014 @default.
• W2047734512 countsByYear W20477345122015 @default.
• W2047734512 countsByYear W20477345122016 @default.
• W2047734512 countsByYear W20477345122017 @default.
• W2047734512 countsByYear W20477345122018 @default.
• W2047734512 countsByYear W20477345122019 @default.
• W2047734512 countsByYear W20477345122020 @default.
• W2047734512 countsByYear W20477345122021 @default.
• W2047734512 countsByYear W20477345122022 @default.
• W2047734512 countsByYear W20477345122023 @default.
• W2047734512 crossrefType "journal-article" @default.
• W2047734512 hasAuthorship W2047734512A5030359755 @default.
• W2047734512 hasAuthorship W2047734512A5042050825 @default.
• W2047734512 hasAuthorship W2047734512A5042190192 @default.
• W2047734512 hasAuthorship W2047734512A5066066688 @default.
• W2047734512 hasConcept C107824862 @default.
• W2047734512 hasConcept C122229402 @default.
• W2047734512 hasConcept C12554922 @default.
• W2047734512 hasConcept C170493617 @default.
• W2047734512 hasConcept C178790620 @default.
• W2047734512 hasConcept C185592680 @default.
• W2047734512 hasConcept C195687474 @default.
• W2047734512 hasConcept C25166345 @default.
• W2047734512 hasConcept C2777339483 @default.
• W2047734512 hasConcept C29688787 @default.
• W2047734512 hasConcept C42086223 @default.
• W2047734512 hasConcept C47701112 @default.
• W2047734512 hasConcept C519063684 @default.
• W2047734512 hasConcept C55493867 @default.
• W2047734512 hasConcept C62614982 @default.
• W2047734512 hasConcept C86803240 @default.
• W2047734512 hasConceptScore W2047734512C107824862 @default.
• W2047734512 hasConceptScore W2047734512C122229402 @default.
• W2047734512 hasConceptScore W2047734512C12554922 @default.
• W2047734512 hasConceptScore W2047734512C170493617 @default.
• W2047734512 hasConceptScore W2047734512C178790620 @default.
• W2047734512 hasConceptScore W2047734512C185592680 @default.
• W2047734512 hasConceptScore W2047734512C195687474 @default.
• W2047734512 hasConceptScore W2047734512C25166345 @default.
• W2047734512 hasConceptScore W2047734512C2777339483 @default.
• W2047734512 hasConceptScore W2047734512C29688787 @default.
• W2047734512 hasConceptScore W2047734512C42086223 @default.
• W2047734512 hasConceptScore W2047734512C47701112 @default.
• W2047734512 hasConceptScore W2047734512C519063684 @default.
• W2047734512 hasConceptScore W2047734512C55493867 @default.
• W2047734512 hasConceptScore W2047734512C62614982 @default.
• W2047734512 hasConceptScore W2047734512C86803240 @default.
• W2047734512 hasIssue "9" @default.
• W2047734512 hasLocation W20477345121 @default.
• W2047734512 hasLocation W20477345122 @default.
• W2047734512 hasOpenAccess W2047734512 @default.
• W2047734512 hasPrimaryLocation W20477345121 @default.
• W2047734512 hasRelatedWork W2011618600 @default.
• W2047734512 hasRelatedWork W2065628212 @default.
• W2047734512 hasRelatedWork W2068219185 @default.
• W2047734512 hasRelatedWork W2080883451 @default.
• W2047734512 hasRelatedWork W2111054934 @default.
• W2047734512 hasRelatedWork W2111813889 @default.
• W2047734512 hasRelatedWork W2120282749 @default.
• W2047734512 hasRelatedWork W2131921914 @default.
• W2047734512 hasRelatedWork W2149893493 @default.
• W2047734512 hasRelatedWork W4240881582 @default.

• next